Department of Science, Faculty of Engineering and Science, Universiti Tunku Abdul Rahman (UTAR), Jalan Genting Kelang, Setapak, 53300 Kuala Lumpur, Malaysia.
Department of Science, Faculty of Engineering and Science, Universiti Tunku Abdul Rahman (UTAR), Jalan Genting Kelang, Setapak, 53300 Kuala Lumpur, Malaysia.
Obes Res Clin Pract. 2010 Oct-Dec;4(4):e247-342. doi: 10.1016/j.orcp.2010.03.002.
Peroxisome proliferator-activated receptors (PPARs) and uncoupling proteins (UCPs) are expressed in adipose tissue, where PPARs are nuclear receptors that function as transcription regulators of the expression of UCPs, mitochondria proteins which uncouple protons in exchange of heat released. The PPARα L162V; PPARγ2 C161T; UCP1 -3826A/G; UCP2 45 bp Ins/Del and -866G/A; and UCP3 -55C/T gene polymorphisms have been previously associated with obesity in different populations, but with inconclusive findings. This study was to investigate the prevalence of these gene polymorphisms and their possible association with obesity in a cohort of Malaysian university students in Kuala Lumpur. Random convenience sampling was performed with informed consents and anthropometric measurements were taken. Mouthwash samples were obtained for genomic DNA extraction and genotyping was performed using Polymerase Chain Reaction - Restriction Fragment Length Polymorphism. Out of the 256 subjects (140 males and 116 females), 170 were lean and 86 were overweight/obese. The mutated PPARα L162V; PPARγ2 C161T; UCP1 -3826A/G; UCP2 45 bp Ins/Del and -866G/A; and UCP3 -55C/T allele frequencies were 0.006, 0.36, 0.58, 0.12, 0.56 and 0.34, respectively. This study failed to find significant differences in the anthropometric indicators of obesity (Body Mass Index, Waist-Hip Ratio and Total Body Fat) between the genotypes of all the PPAR and UCP gene variants. Overall, the genotype and allele distributions were also not significantly different between genders and BMI status. In conclusion, overweight/obesity is not prevalent among the Malaysian university students and the PPAR and UCP gene polymorphisms are not associated with obesity.:
过氧化物酶体增殖物激活受体 (PPARs) 和解偶联蛋白 (UCPs) 在脂肪组织中表达，PPARs 是核受体，作为 UCPs、线粒体蛋白表达的转录调节剂发挥作用，这些线粒体蛋白通过质子交换释放热量。先前已经发现 PPARα L162V；PPARγ2 C161T；UCP1-3826A/G；UCP2 45 bp Ins/Del 和 -866G/A；和 UCP3-55C/T 基因多态性与不同人群的肥胖有关，但结果不一致。本研究旨在调查这些基因多态性在吉隆坡马来西亚大学生队列中的流行情况及其与肥胖的可能关联。采用随机便利抽样，征得知情同意，并进行人体测量。采集漱口样本提取基因组 DNA，采用聚合酶链反应-限制性片段长度多态性进行基因分型。在 256 名受试者中（140 名男性和 116 名女性），170 名受试者为消瘦，86 名受试者为超重/肥胖。突变型 PPARα L162V；PPARγ2 C161T；UCP1-3826A/G；UCP2 45 bp Ins/Del 和 -866G/A；和 UCP3-55C/T 等位基因频率分别为 0.006、0.36、0.58、0.12、0.56 和 0.34。本研究未发现所有 PPAR 和 UCP 基因变异的基因型与肥胖的人体测量指标（体重指数、腰臀比和全身脂肪）存在显著差异。总体而言，性别和 BMI 状态之间的基因型和等位基因分布也没有显著差异。总之，马来西亚大学生超重/肥胖并不普遍，PPAR 和 UCP 基因多态性与肥胖无关。
