Department of Biotechnology, Indian Institute of Technology, Kharagpur, 721302, India.
Integr Biol (Camb). 2014 Feb;6(2):203-14. doi: 10.1039/c3ib40184g.
Disease treatment processes mainly focus on the development of nontoxic, biodegradable, non-immunogenic, biocompatible materials capable of controlled and long-term release of biomolecules. In this work silk protein fibroin from non-mulberry tropical tasar silkworm, Antheraea mylitta, is used to prepare nanoparticles as a drug delivery system. Folate is a vitamin, which is brought into healthy and cancerous cells by folate receptors. The efficiency of silk fibroin-folate nanoparticles loaded with anticancer drug doxorubicin was evaluated by analysing the cell viability, proliferation and endocytosis. Consequently the effects of pro-inflammatory responses by cytokines such as TNF-α, IL-1β and nitric oxide were checked by stimulating the macrophages with folate conjugated silk fibroin nanoparticles. The fibroin-folate nanocarriers are nontoxic, easily taken up by cells and capable of sustained drug release. Nanoparticles loaded with anticancer drug doxorubicin target cancer cells. The results show that silk fibroin-folate nanoparticles may serve as promising nanocarriers for different biomedical and nanotechnology applications in cancer research.
疾病治疗过程主要集中在开发无毒、可生物降解、非免疫原性、生物相容性的材料，这些材料能够控制和长期释放生物分子。在这项工作中，使用来自非桑热带塔萨尔蚕（Antheraea mylitta）的丝蛋白丝素制备纳米颗粒作为药物传递系统。叶酸是一种维生素，它通过叶酸受体被带入健康细胞和癌细胞。通过分析细胞活力、增殖和内吞作用来评估载有抗癌药物阿霉素的丝素-叶酸纳米颗粒的效率。然后通过用叶酸偶联丝素纳米颗粒刺激巨噬细胞来检查细胞因子如 TNF-α、IL-1β 和一氧化氮引起的促炎反应的影响。丝素-叶酸纳米载体无毒，容易被细胞吸收，并能够持续释放药物。载有抗癌药物阿霉素的纳米颗粒靶向癌细胞。结果表明，丝素-叶酸纳米颗粒可作为有前途的纳米载体，用于癌症研究中的不同生物医学和纳米技术应用。
