Wang Juan, Zhang Shanshan, Xing Tieling, Kundu Banani, Li Mingzhong, Kundu Subhas C, Lu Shenzhou
National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou 215123, People's Republic of China.
Department of Biotechnology, Indian Institute of Technology Kharagpur, West Bengal 721302, India.
Int J Biol Macromol. 2015 Aug;79:316-25. doi: 10.1016/j.ijbiomac.2015.04.052. Epub 2015 Apr 30.
Silk protein fibroin in nanoparticles form is a promising material for drug delivery due to its pleiotropic properties, including biocompatibility, biodegradability, ease in fabrication into smaller diameters, high bioavailability, and therapeutic retention at target sites. In the present study, silk nanoparticles are fabricated from regenerated fibroin solution of the Chinese temperate oak tasar Antheraea pernyi by novel ion-induced self-assembly in a very short time under mild conditions. The resultant fibroin nanoparticles range in size from 100 to 500 nm. The molecular conformation of regenerated fibroin changes from α-helical to a β-sheet structure as a rapid function of the ionic strength and the hydrophobic and electrostatic interactions. The mild conditions are potentially advantageous for the encapsulation of sensitive drugs and therapeutic molecules such as doxorubicin hydrochloride, an amphiphilic anticancer therapeutic. In vitro release of doxorubicin from nanoparticles is pH sensitive, with approx. 65% doxorubicin remaining in the fibroin nanoparticles after 11 days. The activity of fibroin nanoparticles on hepatomas indicates the efficacy of the fibroin nanoparticles to maintain the bioactivity of the loaded doxorubicin and impart a dose-dependent cell growth inhibition. The results suggest that Chinese temperate oak tasar silk fibroin nanoparticles can be used as a sustained drug delivery vehicle.
纳米形式的丝蛋白纤维蛋白因其多方面特性，包括生物相容性、生物可降解性、易于制成更小直径、高生物利用度以及在靶位点的治疗保留能力，是一种很有前景的药物递送材料。在本研究中，通过新型离子诱导自组装，在温和条件下于极短时间内由中国温带柞蚕（柞蚕）的再生丝心蛋白溶液制备出丝纳米颗粒。所得的丝心蛋白纳米颗粒尺寸范围为100至500纳米。再生丝心蛋白的分子构象随离子强度以及疏水和静电相互作用而迅速从α螺旋结构转变为β折叠结构。这些温和条件对于封装诸如盐酸多柔比星这种两亲性抗癌治疗药物等敏感药物和治疗分子具有潜在优势。多柔比星从纳米颗粒的体外释放对pH敏感，11天后约65%的多柔比星仍保留在丝心蛋白纳米颗粒中。丝心蛋白纳米颗粒对肝癌的活性表明其能够维持负载的多柔比星的生物活性并产生剂量依赖性的细胞生长抑制作用。结果表明中国温带柞蚕丝心蛋白纳米颗粒可作为一种持续药物递送载体。
