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基于固定在单壁碳纳米管修饰玻碳电极上的酪氨酸酶的生物传感器用于检测肾上腺素。

Biosensor based on tyrosinase immobilized on a single-walled carbon nanotube-modified glassy carbon electrode for detection of epinephrine.

机构信息

Department of Pharmaceutical Sciences, Faculty of Medicine and Pharmacy, Dunarea de Jos University of Galati, Romania.

Department of Chemistry, Physics and Environment, Faculty of Sciences and Environment, Dunarea de Jos University of Galati, Romania.

出版信息

Int J Nanomedicine. 2013;8:4391-8. doi: 10.2147/IJN.S52760. Epub 2013 Nov 12.

DOI:10.2147/IJN.S52760
PMID:24348034
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3848469/
Abstract

A biosensor comprising tyrosinase immobilized on a single-walled carbon nanotube-modified glassy carbon electrode has been developed. The sensitive element, ie, tyrosinase, was immobilized using a drop-and-dry method followed by cross-linking. Tyrosinase maintained high bioactivity on this nanomaterial, catalyzing the oxidation of epinephrine to epinephrine-quinone, which was electrochemically reduced (-0.07 V versus Ag/AgCl) on the biosensor surface. Under optimum conditions, the biosensor showed a linear response in the range of 10-110 μM. The limit of detection was calculated to be 2.54 μM with a correlation coefficient of 0.977. The repeatability, expressed as the relative standard deviation for five consecutive determinations of 10(-5) M epinephrine solution was 3.4%. A good correlation was obtained between results obtained by the biosensor and those obtained by ultraviolet spectrophotometric methods.

摘要

一种基于固定在单壁碳纳米管修饰玻碳电极上的酪氨酸酶的生物传感器已经被开发出来。敏感元件，即酪氨酸酶，通过滴涂-干燥法固定，然后进行交联。在这种纳米材料上，酪氨酸酶保持着很高的生物活性，能够催化儿茶酚的氧化，生成儿茶酚醌，然后在生物传感器表面被电化学还原（相对于 Ag/AgCl 为-0.07 V）。在最佳条件下，该生物传感器在 10-110 μM 的范围内呈现出线性响应。检测限计算为 2.54 μM，相关系数为 0.977。五次连续测定 10(-5) M 肾上腺素溶液的相对标准偏差表示重复性为 3.4%。通过生物传感器获得的结果与通过紫外分光光度法获得的结果之间存在良好的相关性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6b2/3848469/d073f07b8a18/ijn-8-4391Fig7.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6b2/3848469/90d1ede9f580/ijn-8-4391Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6b2/3848469/5cf58681ed58/ijn-8-4391Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6b2/3848469/c7a75a3fad72/ijn-8-4391Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6b2/3848469/d073f07b8a18/ijn-8-4391Fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6b2/3848469/08cb0497466e/ijn-8-4391Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6b2/3848469/1495418c6b30/ijn-8-4391Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6b2/3848469/ecd5e7bfc9f2/ijn-8-4391Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6b2/3848469/90d1ede9f580/ijn-8-4391Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6b2/3848469/5cf58681ed58/ijn-8-4391Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6b2/3848469/c7a75a3fad72/ijn-8-4391Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6b2/3848469/d073f07b8a18/ijn-8-4391Fig7.jpg

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Talanta. 2012 Sep 15;99:270-6. doi: 10.1016/j.talanta.2012.05.050. Epub 2012 Jun 7.
2
Biomimetic sensor for certain catecholamines employing copper(II) complex and silver nanoparticle modified glassy carbon paste electrode.基于铜（II）配合物和银纳米粒子修饰玻碳糊电极的仿生传感器用于特定儿茶酚胺的检测。
Biosens Bioelectron. 2013 Jan 15;39(1):124-32. doi: 10.1016/j.bios.2012.07.008. Epub 2012 Jul 17.
3
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Biosensors (Basel). 2022 Jul 12;12(7):519. doi: 10.3390/bios12070519.
4
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Int J Mol Sci. 2021 Aug 27;22(17):9302. doi: 10.3390/ijms22179302.
5
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9
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4
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5
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Analyst. 2011 Feb 21;136(4):781-6. doi: 10.1039/c0an00379d. Epub 2010 Dec 13.
6
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7
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Biosens Bioelectron. 2010 Oct 15;26(2):798-802. doi: 10.1016/j.bios.2010.06.021. Epub 2010 Jun 25.
8
Aptamers biosensors for pharmaceutical compounds.用于药物化合物的适配体生物传感器。
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9
Adrenaline for the treatment of anaphylaxis: cochrane systematic review.肾上腺素用于治疗过敏反应：Cochrane系统评价
Allergy. 2009 Feb;64(2):204-12. doi: 10.1111/j.1398-9995.2008.01926.x.
10
Piezoelectric detection of ion pairs between sulphonate and catecholamines for flow injection analysis of pharmaceutical preparations.
Talanta. 1999 Mar;48(3):643-8. doi: 10.1016/s0039-9140(98)00287-2.