Research Center for Traditional Chinese Medicine Complexity System, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Pudong, Shanghai 201203, China.
Evid Based Complement Alternat Med. 2013;2013:324732. doi: 10.1155/2013/324732. Epub 2013 Nov 24.
Herbal formulas based on the traditional Chinese medicine (TCM) syndrome (ZHENG) have been used as alternative treatments for breast cancer. However, there is a lack of the experimental animal ZHENG model for the evaluation of the herbal formulas. In this study, we have established 4T1 mouse breast cancer with Liver Fire Invading Stomach Syndrome model (4T1 LFISS mice) and investigated the effects of the herbal formula, Zuo-Jin Wan (ZJW). Our results showed that 4T1 LFISS mice have the features of LFISS including irritability, loss of appetite, yellow urine, chow, and a tail hot. Compared to untreated 4T1 LFISS mice, ZJW significantly reduced tumor weight and volume (P < 0.05), although it was weaker than Cisplatin. However, ZJW significantly increased the body weight and food intake of 4T1 LFISS mice and decreased serum ALT, AST, Cr, and BUN levels and ZHENG score (P < 0.05), while Cisplatin reduced the food intake, and body weight and increased serum ALT, AST, Cr, and BUN levels in 4T1 LFISS mice. Our study has provided a mouse breast cancer ZHENG model and showed that ZJW suppresses tumor growth and improves LFISS and kidney and liver functions in the 4T1 LFISS mice.
基于中医证候（ZHENG）的草药配方已被用作乳腺癌的替代治疗方法。然而，缺乏用于评估草药配方的实验动物 ZHENG 模型。在这项研究中，我们建立了具有肝火犯胃证（4T1 LFISS 小鼠）的 4T1 小鼠乳腺癌模型，并研究了草药配方左金丸（ZJW）的作用。我们的结果表明，4T1 LFISS 小鼠具有 LFISS 的特征，包括烦躁、食欲不振、黄尿、粪便和尾巴热。与未治疗的 4T1 LFISS 小鼠相比，ZJW 显著降低了肿瘤的重量和体积（P<0.05），尽管它比顺铂弱。然而，ZJW 显著增加了 4T1 LFISS 小鼠的体重和采食量，并降低了血清 ALT、AST、Cr 和 BUN 水平和 ZHENG 评分（P<0.05），而顺铂降低了 4T1 LFISS 小鼠的采食量、体重和血清 ALT、AST、Cr 和 BUN 水平。我们的研究提供了一种小鼠乳腺癌 ZHENG 模型，并表明 ZJW 抑制肿瘤生长，改善 4T1 LFISS 小鼠的 LFISS 以及肾脏和肝脏功能。
