Trevor K, Linney E, Oshima R G
Proc Natl Acad Sci U S A. 1987 Feb;84(4):1040-4. doi: 10.1073/pnas.84.4.1040.
Antisense endo B cytokeratin RNA encoded by a retrovirus vector was expressed in a derivative of the F9 embryonal carcinoma cell line. Two G418-resistant clones were selected that expressed a colinear transcript containing both neomycin and antisense endo B cytokeratin sequences. Expression of a 5-fold excess of antisense endo B RNA over endogenous, retinoic acid-induced endo B RNA resulted in suppression of endo B cytokeratin protein expression. In addition, the normal induction of endo A protein, the type II cytokeratin that polymerizes with endo B, was suppressed at the RNA and protein levels. Revertant clones, which synthesize little if any neo or antisense endo B RNA, regain the ability to express the affected gene products in response to retinoic acid. These results indicate that the suppression of endo B cytokeratin protein synthesis influences the stable levels of endo A mRNA.
由逆转录病毒载体编码的反义内切B细胞角蛋白RNA在F9胚胎癌细胞系的一个衍生物中表达。挑选出两个对G418耐药的克隆，它们表达一种共线性转录本，其中包含新霉素和反义内切B细胞角蛋白序列。反义内切B RNA的表达量比内源性视黄酸诱导的内切B RNA过量5倍，导致内切B细胞角蛋白蛋白表达受到抑制。此外，内切A蛋白（与内切B聚合的II型细胞角蛋白）的正常诱导在RNA和蛋白水平均受到抑制。几乎不合成新霉素或反义内切B RNA的回复克隆在视黄酸作用下恢复了表达受影响基因产物的能力。这些结果表明，内切B细胞角蛋白蛋白合成的抑制影响了内切A mRNA的稳定水平。
