Department of Food & Biotechnology, Hanseo University, Seosan, Chungnam 356-706, Korea.
Molecules. 2013 Dec 12;18(12):15474-89. doi: 10.3390/molecules181215474.
Collagen hydrolysate (CH) has been reported to exhibit a positive effect on bone. In the present study, the in vitro effects of CH (<3 kDa) were examined and the in vivo experiments confirmed the positive effects of CH in ovariectomized (OVX) rats. Bone mineral density (BMD) was examined by DXA analysis. Scanning electron microscopic analysis and quantitative 3D-color backscattered electrons imaging analysis were performed on the lumbar vertebrae. CH increased osteoblastic cell proliferation and alkaline phosphatase activity in a dose-dependent manner. Collagen synthesis and collagen, type1, alpha1 (COL1A1) gene expression were also increased by CH treatment. Furthermore, CH-induced COL1A1 gene expression was completely abolished by extracellular signal-regulated kinase (ERK) inhibitor, suggesting the involvement of ERK/MAPK signaling for transcriptional effects on COL1A1 expression. OVX rats supplemented with CH showed osteoprotective effects as the BMD levels were increased compared with control. Moreover, CH prevented the trabecular bone loss induced by OVX and improved the microarchitecture of lumbar vertebrae. CH administration dose-dependently reduced the serum procollagen type I N-terminal propeptide level, which was elevated by OVX. The present study suggests that CH isolated in this study is a promising alternative to current therapeutic agents for the management of osteoporosis.
胶原蛋白水解物（CH）已被报道对骨骼具有积极作用。在本研究中，检测了 CH（<3 kDa）的体外效应，体内实验证实了 CH 对去卵巢（OVX）大鼠的积极影响。通过 DXA 分析检查骨矿物质密度（BMD）。对腰椎进行扫描电子显微镜分析和定量 3D-彩色背散射电子成像分析。CH 以剂量依赖性方式增加成骨细胞增殖和碱性磷酸酶活性。胶原合成和胶原，类型 1，α1（COL1A1）基因表达也被 CH 处理所增加。此外，CH 诱导的 COL1A1 基因表达被细胞外信号调节激酶（ERK）抑制剂完全阻断，表明 ERK/MAPK 信号通路参与了 COL1A1 表达的转录效应。用 CH 补充的 OVX 大鼠表现出骨保护作用，因为 BMD 水平与对照组相比有所增加。此外，CH 可防止 OVX 引起的小梁骨丢失，并改善腰椎的微结构。CH 给药剂量依赖性地降低了血清前胶原 I N 端前肽水平，该水平因 OVX 而升高。本研究表明，本研究中分离的 CH 是目前治疗骨质疏松症的药物的一种有前途的替代物。
