地塞米松对培养的兔关节软骨细胞生长的影响：与核糖皮质激素受体复合物的关系
Effects of dexamethasone on the growth of cultured rabbit articular chondrocytes:relation with the nuclear glucocorticoid-receptor complex.
作者信息
Hainque B, Dominice J, Jaffray P, Ronot X, Adolphe M
出版信息
Ann Rheum Dis. 1987 Feb;46(2):146-52. doi: 10.1136/ard.46.2.146.
Abstract
This study reports that dexamethasone at a high dose (10(-4) mol/l) induced slowing of the in vitro proliferation of rabbit articular chondrocytes in both monolayer and clonal culture. This effect is consistent with an inhibition of DNA and RNA synthesis and was characterised by an accumulation of cells in the G0G1 phase of the cell cycle, as shown by flow cytometric analysis. Therefore we determined the extent of nuclear localisation of dexamethasone-receptor complexes. The results showed a discrepancy between 50% growth inhibitory dose (10(-4) mol/l) and the apparent affinity, KD (1.4 (SD 0.2) X 10(-9) mol/l). Thus the growth inhibition of rabbit articular chondrocytes by dexamethasone did not seem to be related exclusively to an interaction with the glucocorticoid-receptor complexes.
摘要
本研究报告称，高剂量（10⁻⁴mol/L）的地塞米松在单层培养和克隆培养中均能诱导兔关节软骨细胞的体外增殖减缓。这种效应与DNA和RNA合成的抑制一致，并且通过流式细胞术分析表明，其特征是细胞在细胞周期的G0G1期积累。因此，我们测定了地塞米松 - 受体复合物的核定位程度。结果显示，50%生长抑制剂量（10⁻⁴mol/L）与表观亲和力KD（1.4（标准差0.2）×10⁻⁹mol/L）之间存在差异。因此，地塞米松对兔关节软骨细胞的生长抑制似乎并非仅与糖皮质激素 - 受体复合物的相互作用有关。
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