Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan.
Department of Physical Therapy, Graduate Institute of Rehabilitation Science, China Medical University, Taichung, Taiwan.
Chem Biol Interact. 2014 Feb 25;209:77-84. doi: 10.1016/j.cbi.2013.11.017. Epub 2013 Dec 17.
Cardiac apoptosis was found in hearts from hypertensive animals, therefore in this study we aimed to evaluate the anti-apoptotic and pro-survival effects of protocatechuic acid (PCA) on hypertensive hearts. At first we found that, sedentary group (SHR)-PCA group's decreased TUNEL-positive apoptotic cells than SHR group alone. Protein levels of Fas ligand, Fas death receptor, Fas-associated death domain (FADD), Bid, t-Bid, Bax, cytochrome c, activated caspase-8, activated caspase 9 and activated caspase-3 were decreased in SHR-PCA group compared with SHR group. Moreover, SHR-PCA groups increased pro-survival pathway proteins like IGF1, pIGF1R, pPI3K, p-Akt, Bcl-xL, and Bcl-2 than SHR and sedentary normotensive group (WKY). All these finding suggest us that, Protocatechuic acid prevented hypertension-enhanced cardiac Fas-dependent and mitochondria-dependent apoptotic pathways and enhanced cardiac pro-survival pathway in rat models.
心脏细胞凋亡存在于高血压动物的心脏中，因此，本研究旨在评估原儿茶酸（PCA）对高血压心脏的抗凋亡和促生存作用。首先，我们发现，久坐组（SHR）-PCA 组的 TUNEL 阳性凋亡细胞比单独的 SHR 组减少。与 SHR 组相比，SHR-PCA 组的 Fas 配体、Fas 死亡受体、Fas 相关死亡域（FADD）、Bid、t-Bid、Bax、细胞色素 c、活化的 caspase-8、活化的 caspase 9 和活化的 caspase-3 蛋白水平降低。此外，与 SHR 和久坐正常血压组（WKY）相比，SHR-PCA 组的 IGF1、pIGF1R、pPI3K、p-Akt、Bcl-xL 和 Bcl-2 等促生存途径蛋白增加。所有这些发现表明，原儿茶酸可预防高血压增强的心脏 Fas 依赖性和线粒体依赖性凋亡途径，并增强大鼠模型中的心脏促生存途径。
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