原儿茶酸对高血压心脏的抗凋亡和促生存作用。
Anti-apoptotic and pro-survival effect of protocatechuic acid on hypertensive hearts.
机构信息
Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan.
Department of Physical Therapy, Graduate Institute of Rehabilitation Science, China Medical University, Taichung, Taiwan.
出版信息
Chem Biol Interact. 2014 Feb 25;209:77-84. doi: 10.1016/j.cbi.2013.11.017. Epub 2013 Dec 17.
Cardiac apoptosis was found in hearts from hypertensive animals, therefore in this study we aimed to evaluate the anti-apoptotic and pro-survival effects of protocatechuic acid (PCA) on hypertensive hearts. At first we found that, sedentary group (SHR)-PCA group's decreased TUNEL-positive apoptotic cells than SHR group alone. Protein levels of Fas ligand, Fas death receptor, Fas-associated death domain (FADD), Bid, t-Bid, Bax, cytochrome c, activated caspase-8, activated caspase 9 and activated caspase-3 were decreased in SHR-PCA group compared with SHR group. Moreover, SHR-PCA groups increased pro-survival pathway proteins like IGF1, pIGF1R, pPI3K, p-Akt, Bcl-xL, and Bcl-2 than SHR and sedentary normotensive group (WKY). All these finding suggest us that, Protocatechuic acid prevented hypertension-enhanced cardiac Fas-dependent and mitochondria-dependent apoptotic pathways and enhanced cardiac pro-survival pathway in rat models.
心脏细胞凋亡存在于高血压动物的心脏中，因此，本研究旨在评估原儿茶酸（PCA）对高血压心脏的抗凋亡和促生存作用。首先，我们发现，久坐组（SHR）-PCA 组的 TUNEL 阳性凋亡细胞比单独的 SHR 组减少。与 SHR 组相比，SHR-PCA 组的 Fas 配体、Fas 死亡受体、Fas 相关死亡域（FADD）、Bid、t-Bid、Bax、细胞色素 c、活化的 caspase-8、活化的 caspase 9 和活化的 caspase-3 蛋白水平降低。此外，与 SHR 和久坐正常血压组（WKY）相比，SHR-PCA 组的 IGF1、pIGF1R、pPI3K、p-Akt、Bcl-xL 和 Bcl-2 等促生存途径蛋白增加。所有这些发现表明，原儿茶酸可预防高血压增强的心脏 Fas 依赖性和线粒体依赖性凋亡途径，并增强大鼠模型中的心脏促生存途径。
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