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基于集成光学波导的荧光免疫传感器快速灵敏检测湖泊中的微囊藻毒素-LR:优化与分析。

Integrated optical waveguide-based fluorescent immunosensor for fast and sensitive detection of microcystin-LR in lakes: Optimization and Analysis.

机构信息

Center for Sensor Technology of Environment and Health, State Key Joint Laboratory of ESPC, School of Environment, Tsinghua University, Beijing, 10084, China.

Optoelectronics Research Centre, Southampton University, Highfield, Southampton, SO17 1BJ, UK.

出版信息

Sci Rep. 2017 Jun 16;7(1):3655. doi: 10.1038/s41598-017-03939-8.

Abstract

Nowadays, biosensor technologies which can detect various contaminants in water quickly and cost-effectively are in great demand. Herein, we report an integrated channel waveguide-based fluorescent immunosensor with the ability to detect a maximum of 32 contaminants rapidly and simultaneously. In particular, we use waveguide tapers to improve the efficiency of excitation and collection of fluorescent signals in the presence of fluorophore photobleaching in a solid surface bioassay. Under the optimized waveguide geometry, this is the first demonstration of using such a type of waveguide immunosensor for the detection of microcystin-LR (MC-LR) in lake water. The waveguide chip was activated by (3-Mercaptopropyl) trimethoxysilane/N-(4-maleimidobutyryloxy) succinimide (MTS/GMBS) for immobilization of BSA-MC-LR conjugate, which was confirmed to have uniform monolayer distribution by atomic force microscopy. All real lake samples, even those containing MC-LR in the sub-microgram per liter range (e.g. 0.5 μg/L), could be determined by the immunosensor with recovery rates between 84% and 108%, confirming its application potential in the measurement of MC-LR in real water samples.

摘要

如今,能够快速、经济地检测水中各种污染物的生物传感器技术需求量很大。在此,我们报告了一种集成的基于通道波导的荧光免疫传感器,能够快速、同时检测多达 32 种污染物。特别是,我们使用波导渐缩管来提高荧光信号在固体表面生物测定中荧光团光漂白时的激发和收集效率。在优化的波导几何形状下,这是首次使用这种类型的波导免疫传感器来检测湖泊水中的微囊藻毒素-LR(MC-LR)。波导芯片通过(3-巯基丙基)三甲氧基硅烷/N-(4-马来酰亚胺丁酰氧基)琥珀酰亚胺(MTS/GMBS)激活,用于固定 BSA-MC-LR 缀合物,原子力显微镜证实其具有均匀的单层分布。所有真实的湖水样本,甚至那些含有亚微克每升范围(例如 0.5μg/L)的 MC-LR 的样本,都可以通过免疫传感器进行测定,回收率在 84%到 108%之间,证实了其在实际水样中 MC-LR 测量中的应用潜力。

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