Department of Bionano Technology, Hanyang University, Ansan 426-791, South Korea.
Department of Electronics & Communication Engineering, Hanyang University, Ansan 426-791, South Korea.
Biosens Bioelectron. 2015 Feb 15;64:560-5. doi: 10.1016/j.bios.2014.09.087. Epub 2014 Oct 5.
Surface-enhanced Raman scattering (SERS)-based aptasensor platform, using double strand DNA-embedded Au/Ag core-shell nanoparticles, has been developed for the ultrasensitive detection of bisphenol A (BPA) in water. By combining optimally controlled Au/Ag core-shell nanoparticles with the selective BPA binding characteristics of DNA aptamers, a highly sensitive limit of detection (LOD) of 10 fM could be achieved for BPA-spiked tap water over a wide concentration range from 100 nM to 10 fM. This LOD is two or three orders of magnitude lower than that reported for other BPA sensing techniques, and also yields a detection limit that is 100-1000 times lower than the US EPA-defined Predicted No Effect Concentration (PNEC) values in potable water. Total detection time is estimated to be about 40 min including the reaction between aptamer and BPA (30 min) and detection (10 min). This sensing platform is also suitable for field applications since measurement can be performed under aqueous colloidal conditions.
基于表面增强拉曼散射（SERS）的适体传感器平台，使用双链 DNA 嵌入的 Au/Ag 核壳纳米粒子，已被开发用于水中双酚 A（BPA）的超灵敏检测。通过将最佳控制的 Au/Ag 核壳纳米粒子与 DNA 适体的选择性 BPA 结合特性相结合，可以在 100 nM 至 10 fM 的宽浓度范围内实现 BPA 污染自来水的高灵敏度检测限（LOD）为 10 fM。该 LOD 比其他 BPA 传感技术报道的低两个或三个数量级，并且检测限也比美国环保署在饮用水中定义的预测无影响浓度（PNEC）值低 100-1000 倍。包括适体与 BPA 之间的反应（30 分钟）和检测（10 分钟）在内，总检测时间估计约为 40 分钟。由于可以在水胶体条件下进行测量，因此该传感平台也适用于现场应用。
