Li Ying-meng, Fan Xue-mei, Wang Yi-ming, Liang Qiong-lin, Luo Guo-an
School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, China.
Yao Xue Xue Bao. 2013 Sep;48(9):1415-21.
The objective of this study is to fully investigate the therapeutic effect and mechanisms of action of Gegen Qinlian decoction (GD) on type 2 diabetes mellitus (type 2 DM). A rat model of type 2 DM was established with the combination of high-fat diet and multiple low doses of streptozotocin (STZ). Biochemical indicators related to glucose metabolism disorders, insulin resistance, oxidative stress were observed. The type 2 DM rats were administrated with GD for 80 days, the above-mentioned indexes were detected. The results indicated that the hepatic glycogen synthesis level was promoted, fasting blood glucose level and fasting blood insulin level were significantly reduced, insulin sensitivity index was significantly improved; the level of superoxide dismutase (SOD) was increased and the level of malondialdehyde (MDA) was reduced; pathologic morphology of pancreas and kidney was ameliorated in the GD group. It was indicated that the therapeutic mechanisms of action of GD on type 2 DM might be related to its effect of ameliorating glucose metabolism disorders, relieving insulin resistance, increasing the tissues' sensitivity to insulin, improving the antioxidative ability of living system, GD has therapeutic effect on type 2 DM and protective effects against damaged pancreatic function.
本研究的目的是全面探讨葛根芩连汤（GD）对2型糖尿病（2型DM）的治疗作用及作用机制。采用高脂饮食联合多次低剂量链脲佐菌素（STZ）建立2型DM大鼠模型。观察与糖代谢紊乱、胰岛素抵抗、氧化应激相关的生化指标。对2型DM大鼠给予GD灌胃80天，检测上述指标。结果表明，GD可促进肝糖原合成水平，显著降低空腹血糖水平和空腹血胰岛素水平，显著提高胰岛素敏感指数；提高超氧化物歧化酶（SOD）水平，降低丙二醛（MDA）水平；改善GD组胰腺和肾脏的病理形态。提示GD对2型DM的治疗作用机制可能与其改善糖代谢紊乱、缓解胰岛素抵抗、提高组织对胰岛素的敏感性、提高机体抗氧化能力有关，GD对2型DM具有治疗作用及对受损胰腺功能具有保护作用。
