Department of Biology, Faculty of Sciences and Arts, University of Balikesir, Cagis Campus, Balikesir 10145, Turkey.
BMC Complement Altern Med. 2013 Dec 21;13:365. doi: 10.1186/1472-6882-13-365.
The global resurgence of tuberculosis is a significant threat. Lamiaceae members have been used in folk remedies for centuries. This study was designed to assess the in-vitro antimycobacterial activity of eighteen crude extracts from six plants (Lamiaceae) and to characterize their phenolic and flavonoid compounds.
Six Turkish medicinal plants of the family Lamiaceae (Stachys tmolea Boiss., Stachys thirkei C. Koch, Ballota acetabulosa (L.) Benth., Thymus sipthorpii Benth., Satureja aintabensis P.H. Davis, and Micromeria juliana (L.) Benth. ex Reich.) were collected in 2009 - 2010. Dried and crushed plant samples were subjected to sequential extraction with petroleum ether, ethyl acetate, and methanol in order of increasing polarity. A broth microdilution method was employed to screen extracts against four mycobacterial strains of Mycobacterium tuberculosis. Phenolic and flavonoid compounds were characterized by liquid chromatography-mass spectrometry.
S. aintabensis, T. sibthorpii, and M. juliana were found to develop considerable activity against the four strains of M. tuberculosis with the minimal inhibitory concentrations value of 12.5-100 μg/ml. S. aintabensis and T. sibthorpii extracts killed M. tuberculosis with the minimum bactericidal concentration value of 50-800 μg/ml. On the basis of these prominent antimycobacterial activity, we suggest that they could be a source of natural anti-tuberculosis agents.
S. aintabensis and T. sibthorpii showed activity by killing Mycobacteria strains. The major phenolic compound was rosmarinic for T. sibthorpii and S. aintabensis. Flavonoids might be "a modal" for the drug design.
全球结核病的死灰复燃是一个重大威胁。唇形科植物的成员在民间疗法中已经使用了几个世纪。本研究旨在评估六种唇形科植物（Lamiaceae）的 18 种粗提取物的体外抗分枝杆菌活性，并对其酚类和类黄酮化合物进行表征。
2009-2010 年采集了土耳其六种唇形科植物（Stachys tmolea Boiss.、Stachys thirkei C. Koch、Ballota acetabulosa (L.) Benth.、Thymus sipthorpii Benth.、Satureja aintabensis P.H. Davis 和 Micromeria juliana (L.) Benth. ex Reich.）的干燥和粉碎植物样品，依次用石油醚、乙酸乙酯和甲醇进行顺序提取，极性依次增加。采用肉汤微量稀释法筛选提取物对四种结核分枝杆菌株的抑制作用。采用液相色谱-质谱法对酚类和类黄酮化合物进行了表征。
S. aintabensis、T. sibthorpii 和 M. juliana 对四种结核分枝杆菌株均表现出较强的活性，最小抑菌浓度值为 12.5-100μg/ml。S. aintabensis 和 T. sibthorpii 提取物对结核分枝杆菌的最低杀菌浓度值为 50-800μg/ml。基于这些显著的抗分枝杆菌活性，我们认为它们可能是天然抗结核药物的来源。
S. aintabensis 和 T. sibthorpii 通过杀死分枝杆菌菌株显示出活性。主要的酚类化合物是迷迭香酸，对 T. sibthorpii 和 S. aintabensis 均有活性。类黄酮可能是药物设计的“模式”。
