Laboratory of Pharmacognosy (PNBio), Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Rio Grande do Norte, Avenida General Gustavo Cordeiro de Farias, S/N, Petrópolis 59012-570, Natal, Brazil.
Laboratory of Technology and Pharmaceutical Biotechnology (Tecbiofar), Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Rio Grande do Norte, Avenida General Gustavo Cordeiro de Farias, S/N, Petrópolis 59012-570, Natal, Brazil.
Int J Mol Sci. 2018 Feb 24;19(2):636. doi: 10.3390/ijms19020636.
(Apocynaceae) has been popularly used by folk medicine because of its anti-inflammatory, analgesic, and antiophidic properties. This study aims to analyze the flavonoids composition of the hydroethanolic extract and of the ethyl acetate (EtOAc) and butanol (BuOH) fractions of leaves, as well as to evaluate their anti-inflammatory activity using in vivo models. The phytochemical profile, determined by High-Performance Liquid Chromatography-High-Resolution Electrospray Ionization-Mass Spectrometry (HPLC-HRESI-MS), showed the presence of flavonoids mainly having an isorhamnetin nucleus. The anti-inflammatory activity was evaluated in carrageenan-induced paw edema (pre- and post-treatment) with oral administration of a hydroethanolic extract (50, 100, and 150 mg/kg) and of organic fractions (50 mg/kg). The extract and fractions showed antiedematogenic activity by decreasing myeloperoxidase (MPO) production. In the zymosan-air-pouch model, the extract and fractions inhibited leukocyte migration and significantly decreased the levels of various proteins, such as MPO, interleukin (IL)-1β, and tumor necrosis factor (TNF)-α. The cytotoxicity was evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, which revealed no cytotoxicity of the extract and the fractions. These results suggest that the hydroethanolic extract and organic fractions of leaves have sufficient anti-inflammatory activity to support the popular use of this plant in the treatment of inflammatory disorders.
夹竹桃科（Apocynaceae）因其具有抗炎、镇痛和抗蛇毒的特性，在民间医学中被广泛应用。本研究旨在分析叶的水醇提取物以及乙酸乙酯（EtOAc）和正丁醇（BuOH）部分的黄酮类成分，并通过体内模型评估其抗炎活性。通过高效液相色谱-高分辨率电喷雾电离质谱（HPLC-HRESI-MS）确定的植物化学特征表明，存在的黄酮类化合物主要具有异鼠李素核。通过口服水醇提取物（50、100 和 150 mg/kg）和有机部分（50 mg/kg），在角叉菜胶诱导的爪肿胀（预处理和后处理）中评估抗炎活性。提取物和部分通过减少髓过氧化物酶（MPO）的产生显示出抗水肿活性。在酵母聚糖-空气囊模型中，提取物和部分抑制白细胞迁移，并显著降低各种蛋白质（如 MPO、白细胞介素（IL）-1β和肿瘤坏死因子（TNF）-α）的水平。通过 3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐（MTT）测定评估细胞毒性，结果表明提取物和部分没有细胞毒性。这些结果表明，叶的水醇提取物和有机部分具有足够的抗炎活性，支持该植物在治疗炎症性疾病中的广泛应用。
