Petrova I D, Kononova Iu V, Chausov E V, Shestopalov A M, Tishkova F Kh
Mol Gen Mikrobiol Virusol. 2013(3):29-36.
506 Hyalomma anatolicum ticks were collected and assayed in two Crimean-Congo hemorrhagic fever (CCHF) endemic regions of Tajikistan. Antigen and RNA of CCHF virus were detected in 3.4% of tick pools from Rudaki district using ELISA and RT-PCR tests. As of Tursunzade district, viral antigen was identified in 9.0% of samples and viral RNA was identified in 8.1% of samples. The multiple alignment of the obtained nucleotide sequences of CCHF virus genome S-segment 287-nt region (996-1282) and multiple alignment of deduced amino acid sequences of the samples, carried out to compare with CCHF virus strains from the GenBank database, as well as phylogenetic analysis, enabled us to conclude that Asia 1 and Asia 2 genotypes of CCHF virus are circulating in Tajikistan. It is important to note that the genotype Asia 1 virus was detected for the first time in Tajikistan.
在塔吉克斯坦的两个克里米亚-刚果出血热（CCHF）流行地区收集并检测了506只安纳托利亚璃眼蜱。使用酶联免疫吸附测定（ELISA）和逆转录-聚合酶链反应（RT-PCR）检测，在鲁达基区3.4%的蜱虫样本中检测到了CCHF病毒抗原和RNA。在图尔孙扎德区，9.0%的样本中鉴定出病毒抗原，8.1%的样本中鉴定出病毒RNA。对获得的CCHF病毒基因组S片段287核苷酸区域（第⑨⑥-①②⑧②位）的核苷酸序列进行多重比对，并对样本推导的氨基酸序列进行多重比对，以便与来自GenBank数据库的CCHF病毒株进行比较，同时进行系统发育分析，使我们能够得出结论：CCHF病毒的亚洲1型和亚洲2型在塔吉克斯坦传播。需要注意的是，亚洲1型病毒是首次在塔吉克斯坦被检测到。
