2001 - 2003年哈萨克斯坦和塔吉克斯坦克里米亚-刚果出血热病毒的基因监测

[Genetic monitoring of the Crimean-Congo Hemorrhagic Fever virus in Kazakhstan and Tajikistan in 2001-2003].

作者信息

Tumanova I Iu, Seregin S V, Vyshemirskiĭ O I, Gutorov V V, Petrova I D, Tiunnikov G I, Ospanov K S, Kazakov S V, Karimov S, Tishkova F Kh, Pirov A I, Netesov S V, Petrov V S

出版信息

Mol Gen Mikrobiol Virusol. 2006(2):36-41.

PMID:16756002

Abstract

Blood specimens obtained from 32 CCHF patients were tested for the presence of CCHF virus markers. In addition, 3210 ticks of the genera Hyalomma asiaticum, Hyalomma anatolicum, and Dermacentor niveus were examined to identify the CCHF virus antigen and RNA. This material was obtained during the 2001-2003 local outbreaks of CCHF in Kazakhstan and Tajikistan. The nucleotide sequence in the region 983-1282 of S segment of the CCHF virus for 12 wild type strains was determined. The phylogenetic relationships among the established biovariants of CCHF virus, and also between these biovariants and those from other regions of the world were identified. We were the first to demonstrate the presence of an African-like genotype of CCHF virus in the territory of Kazakhstan. The conclusion was made that two genotypes of CCHF virus were in circulation in Kazakhstan. It was also demonstrated that CCHF virus, circulating in the territories of Kazakhstan and Tajikistan, was genetically heterogeneous.

摘要

对32例克里米亚-刚果出血热（CCHF）患者采集的血液样本进行了CCHF病毒标志物检测。此外，还对3210只亚洲璃眼蜱、安纳托利亚璃眼蜱和雪蜱进行了检查，以鉴定CCHF病毒抗原和RNA。这些样本取自2001 - 2003年哈萨克斯坦和塔吉克斯坦当地CCHF疫情期间。测定了12株野生型CCHF病毒S片段983 - 1282区域的核苷酸序列。确定了已确定的CCHF病毒生物变种之间以及这些生物变种与世界其他地区生物变种之间的系统发育关系。我们首次在哈萨克斯坦境内证明存在一种类似非洲基因型的CCHF病毒。得出的结论是，哈萨克斯坦有两种CCHF病毒基因型在传播。还证明，在哈萨克斯坦和塔吉克斯坦境内传播的CCHF病毒在基因上是异质的。