土耳其克里米亚-刚果出血热病毒株M RNA片段的基因分析

Genetic analysis of the M RNA segment of Crimean-Congo hemorrhagic fever virus strains in Turkey.

作者信息

Ozdarendeli A, Aydin K, Tonbak S, Aktas M, Altay K, Koksal I, Bolat Y, Dumanli N, Kalkan A

机构信息

Department of Virology, College of Veterinary Medicine, Firat University, Elazig, Turkey.

出版信息

Arch Virol. 2008;153(1):37-44. doi: 10.1007/s00705-007-1056-4. Epub 2007 Oct 22.

DOI:10.1007/s00705-007-1056-4

PMID:17955162

Abstract

Crimean-Congo hemorrhagic fever (CCHF) virus is member of the genus Nairovirus of the family Bunyaviridae. All members of the family Bunyaviridae are enveloped viruses containing tripartite, negative polarity, single-stranded RNA. CCHF is characterized by high case mortality, occurring in Asia, Africa, the Middle East and Europe. During recent years, outbreaks have been reported in Turkey. However, little information is available on the genetic diversity of CCHF virus in Turkey. In this study, a total of 1227 adult ticks were collected from domestic ruminants (796 specimens from cattle, 399 specimens from goats and 32 specimens from sheep). The presence of the M segment of CCHF virus was determined in 4 of 40 (10%) Hyalomma marginatum marginatum pools, in 2 of 38 (7.89%) Rhipicephalus bursa pools, and in 1 of 7 (7%) Boophylus annulatus pools. Hyalomma anatolicum anatolicum pools gave negative RT-PCR result against CCHF virus. Serum samples from seven patients infected with CCHF were selected and subjected to RT-PCR to amplify partial M segment of CCHF virus. This report introduces the first data on partial nucleotide sequences of M RNA segments of CCHF virus strains circulating in Turkey, isolated from ticks.

摘要

克里米亚-刚果出血热（CCHF）病毒是布尼亚病毒科内罗病毒属的成员。布尼亚病毒科的所有成员都是包膜病毒，含有三条负链单链RNA。CCHF的特点是病死率高，在亚洲、非洲、中东和欧洲均有发生。近年来，土耳其报告了疫情。然而，关于土耳其CCHF病毒的遗传多样性，目前所知信息甚少。在本研究中，共从家养反刍动物身上采集了1227只成年蜱虫（796只来自牛，399只来自山羊，32只来自绵羊）。在40个边缘璃眼蜱（Hyalomma marginatum marginatum）样本池中的4个（10%）、38个柏氏扇头蜱（Rhipicephalus bursa）样本池中的2个（7.89%）以及7个环形牛蜱（Boophylus annulatus）样本池中的1个（7%）中检测到了CCHF病毒的M片段。安纳托利亚璃眼蜱（Hyalomma anatolicum anatolicum）样本池针对CCHF病毒的RT-PCR检测结果为阴性。选取了7例感染CCHF的患者的血清样本，进行RT-PCR以扩增CCHF病毒的部分M片段。本报告介绍了从蜱虫中分离出的、在土耳其传播的CCHF病毒株M RNA片段部分核苷酸序列的首批数据。