Jensen C, Norn S, Thastrup O, Dahl B T, Skov P S
Allergy. 1987 Jan;42(1):51-6. doi: 10.1111/j.1398-9995.1987.tb02187.x.
Histamine release from human basophils was inhibited by preincubation of the cells with a glucolipid mixture containing sialic acid-containing gangliosides. This was true for histamine release induced by anti-IgE, Concanavalin A and the calcium ionophore A23187, whereas the release induced by S. aureus Wood 46 was not affected. It was demonstrated that the inhibitory capacity of the glucolipid mixture could be attributed to the content of gangliosides, since no inhibition was obtained with cerebrosides or with gangliosides from which sialic acid was removed. Preincubation of the cells with the glucolipid mixture increased the sialic acid content of the cells, and this increase was attributed to an insertion of gangliosides into the cell membrane. The inhibition of histamine release was abolished by increasing the calcium concentration, which substantiates our previous findings that cell membrane sialic acid in basophil leukocytes is involved in the regulation of histamine release, possibly by a modulation of the transmembraneous calcium fluxes preceding histamine release.
通过用含有含唾液酸神经节苷脂的糖脂混合物对人嗜碱性粒细胞进行预孵育，可抑制组胺从这些细胞中释放。对于由抗IgE、伴刀豆球蛋白A和钙离子载体A23187诱导的组胺释放而言，情况确实如此，而由金黄色葡萄球菌Wood 46诱导的释放则不受影响。已证明糖脂混合物的抑制能力可归因于神经节苷脂的含量，因为用脑苷脂或去除了唾液酸的神经节苷脂未获得抑制作用。用糖脂混合物对细胞进行预孵育会增加细胞的唾液酸含量，这种增加归因于神经节苷脂插入细胞膜。通过增加钙浓度可消除组胺释放的抑制作用，这证实了我们之前的发现，即嗜碱性白细胞中的细胞膜唾液酸参与组胺释放的调节，可能是通过调节组胺释放之前的跨膜钙通量来实现的。
