Jensen C, Henriksen U, Dahl B T, Skov P S, Norn S
Allergy. 1986 Feb;41(2):151-6. doi: 10.1111/j.1398-9995.1986.tb00291.x.
Since N-acetylneuraminic acid (NANA) in cell membrane glucocalyx mediates or modulates a variety of actions, such as mediator release, we examined a possible modulating role of this amino sugar in histamine release from human basophil leukocytes. Removal of NANA from the cell membrane by the enzyme neuraminidase caused a dose-dependent histamine release. Removal of smaller amounts of NANA enhanced histamine release induced by anti-IgE, Concanavalin A and the calcium ionophore A23187, and reduced the interval between addition of antigen and initiation of histamine release. Pretreatment with free NANA had the opposite effects, i.e. a diminished and delayed maximal histamine release. The hypothesis that NANA in the cell membrane modulates the cellular response to stimulation was further substantiated by demonstrating that the altered response was reflected by a change in the sensitivity of the cell to extracellular calcium. NANA in the cell membrane glucocalyx thus seems to modulate the basophil response to stimulation by modulating transmembraneous calcium transport.
由于细胞膜糖萼中的N-乙酰神经氨酸（NANA）介导或调节多种作用，如介质释放，我们研究了这种氨基糖在人嗜碱性粒细胞组胺释放中可能的调节作用。用神经氨酸酶从细胞膜上去除NANA会导致组胺呈剂量依赖性释放。去除较少量的NANA会增强抗IgE、刀豆球蛋白A和钙离子载体A23187诱导的组胺释放，并缩短添加抗原与组胺释放起始之间的间隔。用游离NANA预处理则产生相反的效果，即最大组胺释放减少且延迟。细胞膜中的NANA调节细胞对刺激的反应这一假说通过以下证明得到进一步证实：细胞对细胞外钙敏感性的变化反映了反应的改变。因此，细胞膜糖萼中的NANA似乎通过调节跨膜钙转运来调节嗜碱性粒细胞对刺激的反应。
