Boezeman J B, Bauer F W, de Grood R M
Cell Tissue Kinet. 1987 Jan;20(1):99-107. doi: 10.1111/j.1365-2184.1987.tb01086.x.
Tape stripping of human skin elicits a proliferative response of a synchronously-dividing group of cells. The progress of this cohort of cells has been monitored using two windows in the cell cycle, one located in mid-S phase and the other centred around G2 + M. The cellular DNA is measured with flow cytometry, the windows are defined by two ranges in the DNA histogram. The cohort can be described as the recruitment of cells from a pre-existing G0 compartment which consists of 76% of all proliferative cells. The duration of the S phase is calculated to be 10.2 hr and G2 + M phase 5.1 hr. The cell cycle time of 39 hr for normal human keratinocytes derived from these figures is in line with recent values obtained by different techniques.
对人体皮肤进行胶带剥离会引发一组同步分裂细胞的增殖反应。已使用细胞周期中的两个窗口监测了这群细胞的进程，一个位于S期中期，另一个以G2 + M期为中心。用流式细胞术测量细胞DNA，通过DNA直方图中的两个范围来定义窗口。这群细胞可描述为从一个预先存在的G0区室募集细胞，该G0区室由所有增殖细胞的76%组成。计算得出S期持续时间为10.2小时，G2 + M期为5.1小时。根据这些数据得出的正常人角质形成细胞的细胞周期时间为39小时，与最近通过不同技术获得的值一致。
