Department of Microbial, Cellular and Molecular Biology, Addis Ababa University, Ethiopia.
Institute of Microbiology, University of Innsbruck, Technikerstraβe 25d, A-6020 Innsbruck, Austria.
Waste Manag. 2014 Mar;34(3):642-52. doi: 10.1016/j.wasman.2013.11.010. Epub 2013 Dec 23.
Various parameters were measured during a 90-day composting process of coffee husk with cow dung (Pile 1), with fruit/vegetable wastes (Pile 2) and coffee husk alone (Pile 3). Samples were collected on days 0, 32 and 90 for chemical and microbiological analyses. C/N ratios of Piles 1 and 2 decreased significantly over the 90 days. The highest bacterial counts at the start of the process and highest actinobacterial counts at the end of the process (Piles 1 and 2) indicated microbial succession with concomitant production of compost relevant enzymes. Denaturing gradient gel electrophoresis of rDNA and COMPOCHIP microarray analysis indicated distinctive community shifts during the composting process, with day 0 samples clustering separately from the 32 and 90-day samples. This study, using a multi-parameter approach, has revealed differences in quality and species diversity of the three composts.
在咖啡壳与牛粪（堆肥 1）、水果/蔬菜废物（堆肥 2）和单独的咖啡壳（堆肥 3）进行为期 90 天的堆肥过程中，测量了各种参数。在第 0、32 和 90 天收集样品进行化学和微生物分析。堆肥 1 和 2 的 C/N 比在 90 天内显著下降。在处理开始时细菌计数最高，在处理结束时放线菌计数最高（堆肥 1 和 2），表明微生物演替伴随着堆肥相关酶的产生。rDNA 变性梯度凝胶电泳和 COMPOCHIP 微阵列分析表明，在堆肥过程中存在独特的群落变化，第 0 天的样品与 32 天和 90 天的样品分开聚类。本研究采用多参数方法，揭示了三种堆肥的质量和物种多样性的差异。
