Zuber P, Losick R
J Bacteriol. 1987 May;169(5):2223-30. doi: 10.1128/jb.169.5.2223-2230.1987.
Transcription of the Bacillus subtilis gene spoVG is induced at the onset of sporulation and is dependent on the products of the stage-0 regulatory genes spo0A, spo0B, and spo0H. We show here that the dependence of spoVG transcription on Spo0A and Spo0B (but not Spo0H) can be bypassed by a mutation at abrB, a previously identified locus at which mutations that suppress some of the phenotypes of spo0A are often located, or by a cis-acting mutation within the spoVG promoter. To explain the epistatis of abrB to spo0A and spo0B mutations, we propose that AbrB acts, directly or indirectly, to block transcription of spoVG and that Spo0A and Spo0B cause inactivation of the abrB gene product(s). Spo0A-Spo0B-dependent inactivation of AbrB could be a general explanation for the pleiotropic effects of spo0A and spo0B mutations on B. subtilis gene expression.
枯草芽孢杆菌基因spoVG的转录在芽孢形成开始时被诱导，并且依赖于0期调控基因spo0A、spo0B和spo0H的产物。我们在此表明，spoVG转录对Spo0A和Spo0B（而非Spo0H）的依赖性可通过abrB处的突变（abrB是先前鉴定的一个位点，抑制spo0A某些表型的突变常常位于此处）或spoVG启动子内的顺式作用突变来绕过。为了解释abrB对spo0A和spo0B突变的上位性，我们提出AbrB直接或间接作用以阻断spoVG的转录，并且Spo0A和Spo0B导致abrB基因产物失活。Spo0A - Spo0B依赖性的AbrB失活可能是spo0A和spo0B突变对枯草芽孢杆菌基因表达产生多效性影响的一个普遍解释。
