Wiest A, Barchers D, Eaton M, Henderson R, Schnittker R, McCluskey K
Fungal Genetics Stock Center, University of Missouri, Kansas City, USA.
J Genet. 2013 Dec;92(3):523-8. doi: 10.1007/s12041-013-0305-4.
Fifteen different classically generated and mapped mutations at the tryptophan synthetase locus in Neurospora crassa have been characterized to the level of the primary sequence of the gene. This sequence analysis has demonstrated that intragenic recombination is accurate to order mutations within one open reading frame. While classic genetic analysis correctly ordered the mutations, the position of mutations characterized by gene sequence analysis was more accurate. A leaky mutation was found to have a wild-type primary sequence. The presence of unique polymorphisms in the primary sequence of the trp-3 gene from strain 861 confirms that it has a unique history relative to the other strains studied. Most strains that were previously shown to be immunologically nonreactive with antibody preparations raised against tryptophan synthetase protein were shown to have nonsense mutations. This work defines 14 alleles of the N. crassa trp-3 gene.
在粗糙脉孢菌色氨酸合成酶基因座上，已经对15个通过经典方法产生并定位的不同突变进行了基因一级序列水平的表征。这种序列分析表明，基因内重组能够精确地排列一个开放阅读框内的突变顺序。虽然经典遗传分析正确地排列了突变顺序，但通过基因序列分析确定的突变位置更为精确。发现一个渗漏突变具有野生型一级序列。来自861菌株的trp - 3基因一级序列中独特多态性的存在证实，相对于所研究的其他菌株，它具有独特的历史。之前显示与针对色氨酸合成酶蛋白制备的抗体制剂无免疫反应的大多数菌株，都被证明具有无义突变。这项工作定义了粗糙脉孢菌trp - 3基因的14个等位基因。
