Institute of Food Chemistry, Technische Universität Braunschweig, Schleinitzstraße 20, 38106 Braunschweig, Germany.
Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), 44270 Guadalajara, JAL, Mexico.
Evid Based Complement Alternat Med. 2013;2013:391247. doi: 10.1155/2013/391247. Epub 2013 Nov 28.
Methanolic avocado (Persea americana Mill., Lauraceae) seed extracts were separated by preparative HSCCC. Partition and HSCCC fractions were principally characterized by LC-ESI-MS/MS analysis. Their in vitro influence was investigated on proliferation, differentiation, cell viability, and gene expression on HaCaT and normal human epidermal keratinocytes (NHEK) and normal human dermal fibroblasts (NHDF). The methanol-water partition (M) from avocado seeds and HSCCC fraction 3 (M.3) were mostly composed of chlorogenic acid and its isomers. Both reduced NHDF but enhanced HaCaT keratinocytes proliferation. HSCCC fraction M.2 composed of quinic acid among chlorogenic acid and its isomers inhibited proliferation and directly induced differentiation of keratinocytes as observed on gene and protein level. Furthermore, M.2 increased NHDF proliferation via upregulation of growth factor receptors. Salidrosides and ABA derivatives present in HSCCC fraction M.6 increased NHDF and keratinocyte proliferation that resulted in differentiation. The residual solvent fraction M.7 contained among low concentrations of ABA derivatives high amounts of proanthocyanidins B1 and B2 as well as an A-type trimer and stimulated proliferation of normal cells and inhibited the proliferation of immortalized HaCaT keratinocytes.
鳄梨（Persea americana Mill.，樟科）的甲醇种子提取物通过制备性 HSCCC 进行分离。分配和 HSCCC 馏分主要通过 LC-ESI-MS/MS 分析进行表征。它们对 HaCaT 和正常人表皮角质形成细胞（NHEK）和正常人真皮成纤维细胞（NHDF）的增殖、分化、细胞活力和基因表达的体外影响进行了研究。鳄梨种子的甲醇-水分配（M）和 HSCCC 馏分 3（M.3）主要由绿原酸及其异构体组成。两者均降低了 NHDF，但增强了 HaCaT 角质形成细胞的增殖。HSCCC 馏分 M.2 由绿原酸及其异构体中的奎尼酸组成，通过基因和蛋白质水平观察到抑制角质形成细胞增殖并直接诱导其分化。此外，M.2 通过上调生长因子受体增加 NHDF 增殖。HSCCC 馏分 M.6 中存在的红景天苷和 ABA 衍生物增加了 NHDF 和角质形成细胞的增殖，导致分化。残留溶剂馏分 M.7 中含有低浓度的 ABA 衍生物，高含量的原花青素 B1 和 B2 以及 A 型三聚体，刺激正常细胞增殖并抑制永生化 HaCaT 角质形成细胞的增殖。
