评估卷尾猴作为宫内节育器对HIV感染影响的模型。
Evaluation of pigtail macaques as a model for the effects of copper intrauterine devices on HIV infection.
作者信息
Engel Robyn M, Morris Monica, Henning Tara, Ritter Jana M, Jones Tara L, Dietz Sharon, Ayers Jessica, Vishwanathan Sundaram A, Jenkins Leecresia, Zaki Sherif, Wildemeersch Dirk, Garber David, Powell Nathaniel, Hendry R Michael, McNicholl Janet, Kersh Ellen N
机构信息
Animal Resources Branch, Division of Scientific Resources, National Center for Emerging and Zoonotic Infectious Diseases, CDC, Atlanta, GA, USA.
出版信息
J Med Primatol. 2014 Oct;43(5):349-59. doi: 10.1111/jmp.12096. Epub 2013 Dec 27.
BACKGROUND
Long-acting, hormonal contraception may increase HIV risk. Copper intrauterine devices (IUDs) could serve as non-hormonal alternatives. We pilot a pigtail macaque model for evaluating HIV susceptibility factors during copper IUD use.
METHODS
Frameless and flexible GyneFix(®) copper IUDs were surgically implanted into three SHIVSF 162p3 -positive macaques via hysterotomy and monitored for up to 4 months. Four macaques served as non-IUD controls.
RESULTS
All animals retained the devices without complications. No consistent change in vaginal viral RNA or inflammatory cytokines was seen. Two animals had altered menstrual cycles and experienced marked thinning of vaginal epithelium after IUD insertion. Histological examination of uterine tissue at necropsy revealed endometrial ulceration and lymphocytic inflammation with glandular loss at sites of direct IUD contact.
CONCLUSIONS
Although the need for insertion surgery could limit its usefulness, this model will allow studies on copper IUDs and SHIV shedding, disease progression, and HIV susceptibility factors.
背景
长效激素避孕可能会增加感染艾滋病毒的风险。铜宫内节育器（IUD）可作为非激素替代品。我们建立了一个卷尾猴模型，用于评估使用铜IUD期间的艾滋病毒易感性因素。
方法
通过剖腹手术将无框架、可弯曲的吉妮柔适（®）铜IUD植入三只感染SHIVSF 162p3的阳性猕猴体内，并监测长达4个月。四只猕猴作为未使用IUD的对照。
结果
所有动物均保留了节育器，无并发症发生。未观察到阴道病毒RNA或炎性细胞因子有持续变化。两只动物的月经周期发生改变，且在插入IUD后阴道上皮明显变薄。尸检时对子宫组织进行的组织学检查显示，在IUD直接接触部位出现子宫内膜溃疡、淋巴细胞炎症以及腺体缺失。
结论
尽管插入手术的必要性可能会限制其应用，但该模型将有助于开展关于铜IUD与SHIV脱落、疾病进展以及艾滋病毒易感性因素的研究。
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