开发一种用于校正直接胆红素测量中溶血的方程。
Development of an equation to correct for hemolysis in direct bilirubin measurements.
机构信息
Kaiser Permanente, TPMG Northern California Regional Laboratory, Berkeley, CA, United States.
University of British Columbia, Department of Pathology and Laboratory Medicine, Vancouver, BC, Canada.
出版信息
Clin Chim Acta. 2014 Feb 15;429:194-7. doi: 10.1016/j.cca.2013.12.023. Epub 2013 Dec 27.
BACKGROUND
Direct bilirubin is measured for the investigation of pediatric and adult jaundice. Package inserts suggest that hemolysis decreases direct bilirubin measurements, but no published studies have adequately described the extent of interference.
METHODS
The influence of hemolysis on direct bilirubin quantification (Beckman AU680) was evaluated by titrating increasing amounts of hemoglobin into specimens with variable starting concentrations of direct bilirubin. An equation was derived to predict the nominal interference-free concentration of direct bilirubin as a function of measured concentration and hemolysis-index.
RESULTS
Hemolysis decreased the direct bilirubin concentration reported by the AU680. The extent of interference is a function of both the interference-free concentration of direct bilirubin and the degree of hemolysis.
CONCLUSIONS
The concentration of direct bilirubin in hemolyzed specimens can be predicted.
背景
直接胆红素用于儿科和成人黄疸的诊断。产品说明书建议溶血会降低直接胆红素的检测值，但目前还没有研究充分描述其干扰程度。
方法
通过向不同起始浓度的直接胆红素标本中逐渐加入血红蛋白来评估溶血对直接胆红素定量（贝克曼 AU680）的影响。推导出一个方程来预测直接胆红素的名义无干扰浓度，作为测量浓度和溶血指数的函数。
结果
溶血降低了 AU680 报告的直接胆红素浓度。干扰的程度取决于直接胆红素的无干扰浓度和溶血程度。
结论
可以预测溶血标本中直接胆红素的浓度。
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