Department of Vascular Surgery, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, PR China.
Department of Vascular Surgery, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, PR China.
Hum Immunol. 2014 Mar;75(3):203-7. doi: 10.1016/j.humimm.2013.12.013. Epub 2013 Dec 27.
Deep venous thrombosis (DVT) and inflammation are two closely related entities. The objective of this study was to evaluate a possible association between interleukin-10 (IL-10) -1082A/G, -819C/T and -592C/A polymorphisms with DVT.
A case-control study was carried out in 660 patients with DVT and 660 age- and gender-matched healthy controls. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) assay was applied to identify the polymorphisms mentioned.
Patients with DVT had a significantly lower frequency of IL-10 -1082GG genotype [odds ratio (OR)=0.59, 95% confidence interval (CI)=0.39, 0.89; P=0.01] than healthy controls. When stratifying by family history of DVT, it was found that patients with positive family history of DVT had a significantly lower frequency of IL-10 -1082GG genotype (OR=0.13, 95% CI=0.02, 0.95; P=0.04). When stratifying by smoking status, presence of varicose veins, type 2 diabetes mellitus and any hormone administration before, no significant differences were found in any groups.
This study provides evidence that IL-10 -1082A/G polymorphism associated with risk of DVT. However, no association of the IL-10 -592C/A or -819C/T polymorphism with DVT risk was found. Additional well-designed large studies were required for the validation of our results.
深静脉血栓形成（DVT）和炎症是两个密切相关的实体。本研究的目的是评估白细胞介素-10（IL-10）-1082A/G、-819C/T 和-592C/A 多态性与 DVT 之间的可能关联。
对 660 例 DVT 患者和 660 名年龄和性别匹配的健康对照进行病例对照研究。应用聚合酶链反应限制性片段长度多态性（PCR-RFLP）分析鉴定上述多态性。
DVT 患者的 IL-10-1082GG 基因型频率明显低于健康对照组[比值比（OR）=0.59，95%置信区间（CI）=0.39，0.89；P=0.01]。按 DVT 家族史分层时，发现有 DVT 家族史的患者 IL-10-1082GG 基因型频率明显较低（OR=0.13，95%CI=0.02，0.95；P=0.04）。按吸烟状况、静脉曲张、2 型糖尿病和任何激素治疗分层时，各组均无显著差异。
本研究提供了证据表明 IL-10-1082A/G 多态性与 DVT 风险相关。然而，IL-10-592C/A 或-819C/T 多态性与 DVT 风险无关联。需要进一步进行设计良好的大型研究来验证我们的结果。
