Cai Feng, Cui Ning, Ma Hongyan, Wang Xueli, Qiao Guihong, Liu Danping
Department of General Surgery, The First People's Hospital of Shangqiu Shangqiu 476100, Henan, China.
Department of Clinical Medicine, Shangqiu Medical College Shangqiu 476100, Henan, China.
Int J Clin Exp Pathol. 2015 Nov 1;8(11):15170-6. eCollection 2015.
We conducted a case-control study to investigate the association between IL-10 gene polymorphism (-1082A/G, -819T/C, and -592A/C) and risk of acute pancreatitis in a Chinese population. A total of 240 patients with proven acute pancreatitis and 240 control subjects were collected between May 2012 and January 2015. Genotyping of the IL-10-1082A/G, -819T/C, and -592A/C gene polymorphisms was conducted by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. By univariate logistic regression analysis, patients with acute pancreatitis were more likely to have higher BMI (OR=2.12, 95% CI=1.45-3.12; P<0.001) and have a habit of alcohol drinking (OR=2.01, 95% CI=1.37-2.95; P<0.001). There were significant differences in the genotype distributions of IL-10-1082A/G between patients with acute pancreatitis and control subjects (χ(2)=9.97, P=0.007). By multiple logistic regression analysis, we found that individuals with the GG genotype of IL-10-1082A/G were associated with an increased risk of acute pancreatitis when compared with the AA genotype (OR=2.32, 95% CI=1.20-4.59; P=0.007). In dominant and recessive models, the IL-10-1082A/G gene polymorphism was significantly correlated with an elevated risk of acute pancreatitis, and the adjusted Ors (95% CI) were 1.50 (1.03-2.20) and 1.99 (1.06-3.79), respectively. However, no significant different was found between IL-10-819T/C and -592A/C gene polymorphisms and susceptibility to acute pancreatitis. In conclusion, we suggest that IL-10-1082A/G gene polymorphisms contribute to the development of acute pancreatitis in codominant, dominant and recessive models.
我们进行了一项病例对照研究，以调查白细胞介素-10（IL-10）基因多态性（-1082A/G、-819T/C和-592A/C）与中国人群急性胰腺炎风险之间的关联。在2012年5月至2015年1月期间，共收集了240例确诊为急性胰腺炎的患者和240例对照者。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法对IL-10 -1082A/G、-819T/C和-592A/C基因多态性进行基因分型。单因素逻辑回归分析显示，急性胰腺炎患者更有可能具有较高的体重指数（BMI）（比值比[OR]=2.12，95%置信区间[CI]=1.45 - 3.12；P<0.001）以及有饮酒习惯（OR=2.01，95% CI=1.37 - 2.95；P<0.001）。急性胰腺炎患者与对照者之间IL-10 -1082A/G基因分型分布存在显著差异（χ(2)=9.97，P=0.007）。多因素逻辑回归分析发现，与AA基因型相比，IL-10 -1082A/G的GG基因型个体患急性胰腺炎的风险增加（OR=2.32，95% CI=1.20 - 4.59；P=0.007）。在显性和隐性模型中，IL-10 -1082A/G基因多态性与急性胰腺炎风险升高显著相关，调整后的比值比（95% CI）分别为1.50（1.03 - 2.20）和1.99（1.06 - 3.79）。然而，未发现IL-10 -819T/C和-592A/C基因多态性与急性胰腺炎易感性之间存在显著差异。总之，我们认为IL-10 -1082A/G基因多态性在共显性、显性和隐性模型中均与急性胰腺炎的发生有关。
