Olurishe Temidayo Olutoyin, Mati Fatima Guiet
Department of Pharmacology and Therapeutics, Ahmadu Bello University, Zaria PMB 1045, Zaria, Nigeria.
Pak J Pharm Sci. 2014 Jan;27(1):169-72.
The ethnomedical uses of Laggera aurita (LA), including its anti-nociceptive properties have been documented in literature. This study evaluated the anti-hyperalgesic effects of the methanolic extract of LA (MELA) using chemically and mechanically induced hyperalgesia models. Acute toxicity and preliminary phytochemical screening were also conducted. The intraperitoneal median lethal dose was found to be 3807.88 mg/kg, while saponins, tannins and carbohydrates were found to be present in the plant material. MELA exhibited significant analgesic activity in the acetic acid induced writhing and Randall-Siletto tests. The 400 mg/kg dose of MELA exhibited a significant (p<0.001) analgesic activity which offered 19.75% inhibition above piroxicam in the acetic acid test. At 200 and 400 mg/kg MELA demonstrated comparative analgesia with pentazocine in the Randall-Siletto test. The study shows that MELA possesses anti-nociceptive principles and the presence of saponins, tannins and carbohydrates which have been previously associated with anti-hyperalgesia may be responsible for the pharmacological actions, thus authenticating the ethnomedical rationale for its anti-nociceptive uses.
文献中已记载了六耳铃的民族医学用途，包括其抗伤害感受特性。本研究使用化学和机械诱导的痛觉过敏模型评估了六耳铃甲醇提取物（MELA）的抗痛觉过敏作用。还进行了急性毒性试验和初步的植物化学筛选。腹腔注射的半数致死剂量为3807.88毫克/千克，同时发现植物材料中存在皂苷、单宁和碳水化合物。MELA在醋酸诱导的扭体试验和兰德尔-西莱托试验中表现出显著的镇痛活性。400毫克/千克剂量的MELA表现出显著（p<0.001）的镇痛活性，在醋酸试验中比吡罗昔康的抑制率高19.75%。在兰德尔-西莱托试验中，200和400毫克/千克的MELA与喷他佐辛表现出相当的镇痛效果。该研究表明，MELA具有抗伤害感受的成分，先前与抗痛觉过敏相关的皂苷、单宁和碳水化合物的存在可能是其药理作用的原因，从而证实了其抗伤害感受用途的民族医学原理。
