Arai Hirofumi
Department of Biotechnology and Environmental Chemistry, Kitami Institute of Technology, Kitami, Hokkaido, 090-8507, Japan,
Subcell Biochem. 2014;77:103-14. doi: 10.1007/978-94-007-7920-4_9.
Lipoproteins consist of lipids and apolipoproteins that have functional roles in lipid metabolism. It has been suggested that oxidation of lipoproteins by reactive oxygen species (ROS) may be involved in the inception of various diseases. In particular, the relationship between low-density lipoprotein (LDL) oxidation and atherosclerosis has been studied in great detail. The main target molecules of lipoprotein oxidation are polyunsaturated fatty acid residues of lipids and apolipoproteins. Extensive investigations have characterized oxidative modifications of apolipoprotein B100 (apo B100) in LDL. Furthermore, modifications of apo B100 by oxidized lipids have been confirmed in oxidized LDL and atherosclerotic lesions using immunological techniques. In this chapter, characteristics and oxidation mechanisms of lipoproteins by ROS are described from in vitro and in vivo studies. Oxidative modifications of apo B100 by lipid hydroperoxides, major products of lipid peroxidation at the early stage, are principally reported.
脂蛋白由脂质和载脂蛋白组成，它们在脂质代谢中发挥着功能性作用。有研究表明，活性氧（ROS）对脂蛋白的氧化作用可能与多种疾病的发生有关。特别是，低密度脂蛋白（LDL）氧化与动脉粥样硬化之间的关系已得到深入研究。脂蛋白氧化的主要靶分子是脂质和载脂蛋白中的多不饱和脂肪酸残基。大量研究已对LDL中载脂蛋白B100（apo B100）的氧化修饰进行了表征。此外，利用免疫学技术已在氧化LDL和动脉粥样硬化病变中证实了氧化脂质对apo B100的修饰。在本章中，将从体外和体内研究描述ROS对脂蛋白的特性和氧化机制。主要报道了脂质过氧化早期主要产物脂质氢过氧化物对apo B100的氧化修饰。
