Almajhdi Fahad N, Fouad H, Khalil Khalil Abdelrazek, Awad Hanem M, Mohamed Sahar H S, Elsarnagawy T, Albarrag Ahmed M, Al-Jassir Fawzi F, Abdo Hany S
Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia.
J Mater Sci Mater Med. 2014 Apr;25(4):1045-53. doi: 10.1007/s10856-013-5131-y. Epub 2013 Dec 31.
In the present work, a series of 0, 1 and 7 wt% silver nano-particles (Ag NPs) incorporated poly lactic-co-glycolic acid (PLGA) nano-fibers were synthesized by the electrospinning process. The PLGA/Ag nano-fibers sheets were characterized using SEM, TEM and DSC analyses. The three synthesized PLGA/silver nano-fiber composites were screened for anticancer activity against liver cancer cell line using MTT and LDH assays. The anticancer activity of PLGA nano-fibers showed a remarkable improvement due to increasing the concentration of the Ag NPs. In addition to the given result, PLGA nano-fibers did not show any cytotoxic effect. However, PLGA nano-fibers that contain 1 % nano silver showed anticancer activity of 8.8 %, through increasing the concentration of the nano silver to 7 % onto PLGA nano-fibers, the anticancer activity was enhanced to a 67.6 %. Furthermore, the antibacterial activities of these three nano-fibers, against the five bacteria strains namely; E.coli o157:H7 ATCC 51659, Staphylococcus aureus ATCC 13565, Bacillus cereus EMCC 1080, Listeria monocytogenes EMCC 1875 and Salmonella typhimurium ATCC25566 using the disc diffusion method, were evaluated. Sample with an enhanced inhibitory effect was PLGA/Ag NPs (7 %) which inhibited all strains (inhibition zone diameter 10 mm); PLGA/Ag NPs (1 %) sample inhibited only one strain (B. cereus) with zone diameter 8 mm. The PLGA nano-fiber sample has not shown any antimicrobial activity. Based on the anticancer as well as the antimicrobial results in this study, it can be postulated that: PLGA nanofibers containing 7 % nano silver are suitable as anticancer- and antibiotic-drug delivery systems, as they will increase the anticancer as well as the antibiotic drug potency without cytotoxicity effect on the normal cells. These findings also suggest that Ag NPs, of the size (5-10 nm) evaluated in the present study, are appropriate for therapeutic application from a safety standpoint.
在本研究中，通过静电纺丝工艺合成了一系列分别含有0、1和7 wt%银纳米颗粒（Ag NPs）的聚乳酸-乙醇酸共聚物（PLGA）纳米纤维。使用扫描电子显微镜（SEM）、透射电子显微镜（TEM）和差示扫描量热法（DSC）对PLGA/Ag纳米纤维片进行了表征。使用MTT和LDH测定法筛选了三种合成的PLGA/银纳米纤维复合材料对肝癌细胞系的抗癌活性。由于Ag NPs浓度的增加，PLGA纳米纤维的抗癌活性有了显著提高。除了给出的结果外，PLGA纳米纤维没有显示出任何细胞毒性作用。然而，含有1%纳米银的PLGA纳米纤维显示出8.8%的抗癌活性，通过将PLGA纳米纤维上的纳米银浓度提高到7%，抗癌活性提高到了67.6%。此外，使用纸片扩散法评估了这三种纳米纤维对五种细菌菌株的抗菌活性，这五种细菌菌株分别是：大肠杆菌o157:H7 ATCC 51659、金黄色葡萄球菌ATCC 13565、蜡样芽孢杆菌EMCC 1080、单核细胞增生李斯特菌EMCC 1875和鼠伤寒沙门氏菌ATCC25566。具有增强抑制作用的样品是PLGA/Ag NPs（7%），它抑制了所有菌株（抑菌圈直径为10 mm）；PLGA/Ag NPs（1%）样品仅抑制了一种菌株（蜡样芽孢杆菌），抑菌圈直径为8 mm。PLGA纳米纤维样品没有显示出任何抗菌活性。基于本研究中的抗癌和抗菌结果，可以推测：含有7%纳米银的PLGA纳米纤维适合作为抗癌和抗生素药物递送系统，因为它们将提高抗癌和抗生素药物的效力，而对正常细胞没有细胞毒性作用。这些发现还表明，从安全角度来看，本研究中评估的尺寸（5-10 nm）的Ag NPs适用于治疗应用。
