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大肠杆菌30S核糖体亚基中的RNA-蛋白质交联；通过用双（2-氯乙基）甲胺处理确定16S RNA上与蛋白质S3、S4、S7、S9、S10、S11、S17、S18和S21交联的位点。

RNA-protein cross-linking in Escherichia coli 30S ribosomal subunits; determination of sites on 16S RNA that are cross-linked to proteins S3, S4, S7, S9, S10, S11, S17, S18 and S21 by treatment with bis-(2-chloroethyl)-methylamine.

作者信息

Greuer B, Osswald M, Brimacombe R, Stöffler G

出版信息

Nucleic Acids Res. 1987 Apr 24;15(8):3241-55. doi: 10.1093/nar/15.8.3241.

DOI:10.1093/nar/15.8.3241
PMID:2437528
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC340727/
Abstract

RNA-protein cross-links were introduced into E. coli 30S ribosomal subunits by treatment with bis-(2-chloroethyl)-methylamine. After partial nuclease digestion of the RNA moiety, a number of cross-linked RNA-protein complexes were isolated by a new three-step procedure. Protein and RNA analysis of the individual complexes gave the following results: proteins S4 and S9 are cross-linked to the 16S RNA at positions 413 and 954, respectively. Proteins S11 and S21 are both cross-linked to the RNA within an oligonucleotide encompassing positions 693-697, and proteins S17, S10, S3 and S7 are cross-linked within oligonucleotides encompassing positions 278-280, 1139-1144, 1155-1158, and 1531-1542, respectively. A cross-link to protein S18 was found by a process of elimination to lie between positions 845 and 851.

摘要

通过用双（2-氯乙基）甲胺处理，将RNA-蛋白质交联引入大肠杆菌30S核糖体亚基。在对RNA部分进行部分核酸酶消化后，通过一种新的三步程序分离出许多交联的RNA-蛋白质复合物。对各个复合物的蛋白质和RNA分析得出以下结果：蛋白质S4和S9分别在位置413和954与16S RNA交联。蛋白质S11和S21都与包含位置693 - 697的寡核苷酸内的RNA交联，蛋白质S17、S10、S3和S7分别在包含位置278 - 280、1139 - 1144、1155 - 1158和1531 - 1542的寡核苷酸内交联。通过排除法发现与蛋白质S18的交联位于位置845和851之间。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9787/340727/78c6063cc17c/nar00252-0060-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9787/340727/d4cbae77bba5/nar00252-0058-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9787/340727/ec9e9a24fe1c/nar00252-0059-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9787/340727/78c6063cc17c/nar00252-0060-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9787/340727/d4cbae77bba5/nar00252-0058-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9787/340727/ec9e9a24fe1c/nar00252-0059-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9787/340727/78c6063cc17c/nar00252-0060-a.jpg

相似文献

1
RNA-protein cross-linking in Escherichia coli 30S ribosomal subunits; determination of sites on 16S RNA that are cross-linked to proteins S3, S4, S7, S9, S10, S11, S17, S18 and S21 by treatment with bis-(2-chloroethyl)-methylamine.大肠杆菌30S核糖体亚基中的RNA-蛋白质交联；通过用双（2-氯乙基）甲胺处理确定16S RNA上与蛋白质S3、S4、S7、S9、S10、S11、S17、S18和S21交联的位点。
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The use of azidoarylimidoesters in RNA-protein cross-linking studies with Escherichia coli ribosomes.叠氮芳基酰亚胺酯在大肠杆菌核糖体的RNA-蛋白质交联研究中的应用。
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Precise localisation of three intra-RNA cross-links in 23S RNA and one in 5S RNA, induced by treatment of Escherichia coli 50S ribosomal subunits with bis-(2-chloroethyl)-methylamine.用双（2-氯乙基）甲胺处理大肠杆菌50S核糖体亚基后，在23S RNA中诱导产生的三个RNA内交联以及在5S RNA中诱导产生的一个交联的精确定位。
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春雷霉素诱导的一种意外类型的核糖体：窥探蛋白质合成的远古时代？
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Crosslinking of ribosomal proteins to RNA in maize ribosomes by UV-B and its effects on translation.紫外线B对玉米核糖体中核糖体蛋白与RNA的交联作用及其对翻译的影响。
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The identification of spermine binding sites in 16S rRNA allows interpretation of the spermine effect on ribosomal 30S subunit functions.在16S核糖体RNA中精胺结合位点的鉴定有助于解释精胺对核糖体30S亚基功能的影响。
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大肠杆菌16S和23S核糖体RNA的精细二级结构模型
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5
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Mol Gen Genet. 1972;119(4):357-66.
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