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大肠杆菌30S核糖体亚基中的RNA-蛋白质交联；通过对甲基对叠氮基苯乙酰亚胺处理，确定16S RNA上与蛋白质S3、S4、S5、S7、S8、S9、S11、S13、S19和S21交联的位点。

RNA-protein cross-linking in Escherichia coli 30S ribosomal subunits; determination of sites on 16S RNA that are cross-linked to proteins S3, S4, S5, S7, S8, S9, S11, S13, S19 and S21 by treatment with methyl p-azidophenyl acetimidate.

作者信息

Osswald M, Greuer B, Brimacombe R, Stöffler G, Bäumert H, Fasold H

出版信息

Nucleic Acids Res. 1987 Apr 24;15(8):3221-40. doi: 10.1093/nar/15.8.3221.

DOI:10.1093/nar/15.8.3221
PMID:2437527
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC340726/
Abstract

RNA-protein cross-links were introduced into E. coli 30S ribosomal subunits by treatment with methyl p-azidophenyl acetimidate. After partial nuclease digestion of the RNA moiety, a number of cross-linked RNA-protein complexes were isolated by a new three-step procedure. Protein and RNA analysis of the individual complexes gave the following results: Proteins S3, S4, S5 and S8 are cross-linked to the 5'-terminal tetranucleotide of 16S RNA. S5 is also cross-linked to the 16S RNA within an oligonucleotide encompassing positions 559-561. Proteins S11, S9, S19 and S7 are cross-linked to 16S RNA within oligonucleotides encompassing positions 702-705, 1130-1131, 1223-1231 and 1238-1240, respectively. Protein S13 is cross-linked to an oligonucleotide encompassing positions 1337-1338, and is also involved in an anomalous cross-link within positions 189-191. Protein S21 is cross-linked to the 3'-terminal dodecanucleotide of the 16S RNA.

摘要

通过用对叠氮苯基乙酰亚氨酸甲酯处理，将RNA - 蛋白质交联引入大肠杆菌30S核糖体亚基。在对RNA部分进行部分核酸酶消化后，通过一种新的三步程序分离出许多交联的RNA - 蛋白质复合物。对各个复合物的蛋白质和RNA分析得出以下结果：蛋白质S3、S4、S5和S8与16S RNA的5'-末端四核苷酸交联。S5还与包含位置559 - 561的寡核苷酸内的16S RNA交联。蛋白质S11、S9、S19和S7分别与包含位置702 - 705、1130 - 1131、1223 - 1231和1238 - 1240的寡核苷酸内的16S RNA交联。蛋白质S13与包含位置1

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f239/340726/1a9f20fbda5d/nar00252-0044-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f239/340726/c8a9221edf59/nar00252-0041-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f239/340726/166bca1049f5/nar00252-0043-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f239/340726/1a9f20fbda5d/nar00252-0044-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f239/340726/c8a9221edf59/nar00252-0041-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f239/340726/166bca1049f5/nar00252-0043-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f239/340726/1a9f20fbda5d/nar00252-0044-a.jpg

相似文献

1
RNA-protein cross-linking in Escherichia coli 30S ribosomal subunits; determination of sites on 16S RNA that are cross-linked to proteins S3, S4, S5, S7, S8, S9, S11, S13, S19 and S21 by treatment with methyl p-azidophenyl acetimidate.大肠杆菌30S核糖体亚基中的RNA-蛋白质交联；通过对甲基对叠氮基苯乙酰亚胺处理，确定16S RNA上与蛋白质S3、S4、S5、S7、S8、S9、S11、S13、S19和S21交联的位点。
Nucleic Acids Res. 1987 Apr 24;15(8):3221-40. doi: 10.1093/nar/15.8.3221.
2
RNA-protein cross-linking in Escherichia coli 30S ribosomal subunits; determination of sites on 16S RNA that are cross-linked to proteins S3, S4, S7, S9, S10, S11, S17, S18 and S21 by treatment with bis-(2-chloroethyl)-methylamine.大肠杆菌30S核糖体亚基中的RNA-蛋白质交联；通过用双（2-氯乙基）甲胺处理确定16S RNA上与蛋白质S3、S4、S7、S9、S10、S11、S17、S18和S21交联的位点。
Nucleic Acids Res. 1987 Apr 24;15(8):3241-55. doi: 10.1093/nar/15.8.3241.
3
The localization of multiple sites on 16S RNA which are cross-linked to proteins S7 and S8 in Escherichia coli 30S ribosomal subunits by treatment with 2-iminothiolane.通过用2-亚氨基硫杂环戊烷处理，对大肠杆菌30S核糖体亚基中与蛋白质S7和S8交联的16S RNA上多个位点的定位。
Nucleic Acids Res. 1983 Mar 11;11(5):1419-37. doi: 10.1093/nar/11.5.1419.
4
RNA-protein cross-linking in Escherichia coli ribosomal subunits: localization of sites on 16S RNA which are cross-linked to proteins S17 and S21 by treatment with 2-iminothiolane.大肠杆菌核糖体亚基中的RNA-蛋白质交联：通过用2-亚氨基硫杂环戊烷处理，定位16S RNA上与蛋白质S17和S21交联的位点。
Nucleic Acids Res. 1986 Feb 11;14(3):1171-86. doi: 10.1093/nar/14.3.1171.
5
Multiple crosslinks of proteins S7 and S9 to domains 3 and 4 of 16S ribosomal RNA in the Escherichia coli 30S particle.蛋白质S7和S9与大肠杆菌30S颗粒中16S核糖体RNA的结构域3和结构域4的多个交联。
Eur J Biochem. 1986 Oct 15;160(2):363-70. doi: 10.1111/j.1432-1033.1986.tb09980.x.
6
Chemical cross-linking of protein to RNA within intact ribosomal subunits from Escherichia coli.蛋白质与来自大肠杆菌的完整核糖体亚基内的RNA的化学交联
Mol Gen Genet. 1978 Apr 6;160(2):183-93. doi: 10.1007/BF00267480.
7
Synthesis of a new reagent, ethyl 4-azidobenzoylaminoacetimidate, and its use for RNA-protein cross-linking within Escherichia coli ribosomal 30-S subunits.一种新试剂4-叠氮基苯甲酰氨基乙酸乙酯的合成及其在大肠杆菌核糖体30-S亚基内用于RNA-蛋白质交联的应用。
Eur J Biochem. 1980 Sep;110(2):485-92. doi: 10.1111/j.1432-1033.1980.tb04890.x.
8
[Contacts of ribosomal proteins with tRNAPhe and 16S RNA in analogs of the 30S initiation complex].[核糖体蛋白与30S起始复合物类似物中苯丙氨酰tRNA及16S RNA的相互作用]
Mol Biol (Mosk). 1985 Mar-Apr;19(2):553-7.
9
Efficient reconstitution of functional Escherichia coli 30S ribosomal subunits from a complete set of recombinant small subunit ribosomal proteins.利用一整套重组小亚基核糖体蛋白高效重建功能性大肠杆菌30S核糖体亚基。
RNA. 1999 Jun;5(6):832-43. doi: 10.1017/s1355838299990714.
10
Crosslinking of ribosomal proteins S4, S5, S7, S8, S11, S12 and S18 to domains 1 and 2 of 16S rRNA in the Escherichia coli 30S particle.核糖体蛋白S4、S5、S7、S8、S11、S12和S18与大肠杆菌30S颗粒中16S rRNA的结构域1和结构域2的交联。
Biochimie. 1989 Jul;71(7):839-52. doi: 10.1016/0300-9084(89)90048-5.

引用本文的文献

1
Identification of Novel RNA-Protein Contact in Complex of Ribosomal Protein S7 and 3'-Terminal Fragment of 16S rRNA in E. coli.鉴定大肠杆菌核糖体蛋白 S7 与 16S rRNA 3'末端片段复合物中的新型 RNA-蛋白质接触点。
Acta Naturae. 2012 Oct;4(4):65-72.
2
Slow formation of stable complexes during coincubation of minimal rRNA and ribosomal protein S4.在最小 rRNA 和核糖体蛋白 S4 共孵育过程中稳定复合物的缓慢形成。
J Mol Biol. 2011 Sep 23;412(3):453-65. doi: 10.1016/j.jmb.2011.07.048. Epub 2011 Jul 29.
3
Mining biochemical information: lessons taught by the ribosome.

本文引用的文献

1
The use of azidoarylimidoesters in RNA-protein cross-linking studies with Escherichia coli ribosomes.叠氮芳基酰亚胺酯在大肠杆菌核糖体的RNA-蛋白质交联研究中的应用。
J Mol Biol. 1980 Mar 5;137(3):301-4. doi: 10.1016/0022-2836(80)90318-6.
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An improved method for two-dimensional gel-electrophoresis: analysis of mutationally altered ribosomal proteins of Escherichia coli.一种改进的二维凝胶电泳方法：大肠杆菌突变核糖体蛋白的分析
Mol Gen Genet. 1981;181(3):309-12. doi: 10.1007/BF00425603.
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Reagents suitable for the crosslinking of nucleic acids to proteins.
挖掘生化信息：核糖体带来的启示
RNA. 2002 Mar;8(3):279-89. doi: 10.1017/s135583820202407x.
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A novel ribosome-associated protein is important for efficient translation in Escherichia coli.一种新型核糖体相关蛋白对大肠杆菌中的高效翻译很重要。
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Genetic and comparative analyses reveal an alternative secondary structure in the region of nt 912 of Escherichia coli 16S rRNA.遗传分析和比较分析揭示了大肠杆菌16S rRNA第912位核苷酸区域的一种替代性二级结构。
Proc Natl Acad Sci U S A. 1995 Nov 7;92(23):10555-9. doi: 10.1073/pnas.92.23.10555.
6
Covalent cross-linking of poly(A) to Escherichia coli ribosomes, and localization of the cross-link site within the 16S RNA.聚腺苷酸与大肠杆菌核糖体的共价交联，以及交联位点在16S RNA中的定位。
Nucleic Acids Res. 1988 Mar 25;16(6):2369-88. doi: 10.1093/nar/16.6.2369.
7
Protein binding sites on Escherichia coli 16S ribosomal RNA; RNA regions that are protected by proteins S7, S9 and S19, and by proteins S8, S15 and S17.大肠杆菌16S核糖体RNA上的蛋白质结合位点；受蛋白质S7、S9和S19以及蛋白质S8、S15和S17保护的RNA区域。
Nucleic Acids Res. 1988 Feb 25;16(4):1233-50. doi: 10.1093/nar/16.4.1233.
8
The Escherichia coli 30S ribosomal subunit; an optimized three-dimensional fit between the ribosomal proteins and the 16S RNA.大肠杆菌30S核糖体亚基；核糖体蛋白与16S RNA之间优化的三维契合。
EMBO J. 1988 May;7(5):1509-13. doi: 10.1002/j.1460-2075.1988.tb02970.x.
9
The E. coli 16S rRNA binding site of ribosomal protein S15: higher-order structure in the absence and in the presence of the protein.核糖体蛋白S15的大肠杆菌16S rRNA结合位点：在无蛋白和有蛋白情况下的高级结构
Nucleic Acids Res. 1988 Apr 11;16(7):2825-39. doi: 10.1093/nar/16.7.2825.
10
Localization of a series of RNA-protein cross-link sites in the 23S and 5S ribosomal RNA from Escherichia coli, induced by treatment of 50S subunits with three different bifunctional reagents.
Nucleic Acids Res. 1990 Dec 11;18(23):6755-60. doi: 10.1093/nar/18.23.6755.
适用于核酸与蛋白质交联的试剂。
Anal Biochem. 1980 Nov 1;108(2):394-401. doi: 10.1016/0003-2697(80)90604-1.
4
Precise localization of the site of cross-linking between protein L4 and 23S ribonucleic acid induced by mild ultraviolet irradiation of Escherichia coli 50S ribosomal subunits.大肠杆菌50S核糖体亚基经轻度紫外线照射后，蛋白质L4与23S核糖核酸之间交联位点的精确定位。
Biochemistry. 1980 Sep 2;19(18):4179-88. doi: 10.1021/bi00559a007.
5
Precise localisation of three intra-RNA cross-links in 23S RNA and one in 5S RNA, induced by treatment of Escherichia coli 50S ribosomal subunits with bis-(2-chloroethyl)-methylamine.用双（2-氯乙基）甲胺处理大肠杆菌50S核糖体亚基后，在23S RNA中诱导产生的三个RNA内交联以及在5S RNA中诱导产生的一个交联的精确定位。
Nucleic Acids Res. 1982 Nov 25;10(22):7211-29. doi: 10.1093/nar/10.22.7211.
6
Refined secondary structure models for the 16S and 23S ribosomal RNA of Escherichia coli.大肠杆菌16S和23S核糖体RNA的精细二级结构模型
Nucleic Acids Res. 1983 Nov 11;11(21):7263-86. doi: 10.1093/nar/11.21.7263.
7
The localization of multiple sites on 16S RNA which are cross-linked to proteins S7 and S8 in Escherichia coli 30S ribosomal subunits by treatment with 2-iminothiolane.通过用2-亚氨基硫杂环戊烷处理，对大肠杆菌30S核糖体亚基中与蛋白质S7和S8交联的16S RNA上多个位点的定位。
Nucleic Acids Res. 1983 Mar 11;11(5):1419-37. doi: 10.1093/nar/11.5.1419.
8
Topography of 16 S RNA in 30 S subunits and 70 S ribosomes accessibility to cobra venom ribonuclease.30S亚基和70S核糖体中16S RNA的拓扑结构：对眼镜蛇毒核糖核酸酶的可及性
J Mol Biol. 1981 Nov 15;152(4):699-721. doi: 10.1016/0022-2836(81)90123-6.
9
The use of 2-iminothiolane as an RNA-protein cross-linking agent in Escherichia coli ribosomes, and the localisation on 23S RNA of sites cross-linked to proteins L4, L6, L21, L23, L27 and L29.2-亚氨基硫杂环戊烷作为RNA-蛋白质交联剂在大肠杆菌核糖体中的应用，以及与蛋白质L4、L6、L21、L23、L27和L29交联位点在23S RNA上的定位。
Nucleic Acids Res. 1981 Sep 11;9(17):4285-302. doi: 10.1093/nar/9.17.4285.
10
Inactivation and reactivation of ribosomal subunits: amino acyl-transfer RNA binding activity of the 30 s subunit of Escherichia coli.核糖体亚基的失活与重新激活：大肠杆菌30S亚基的氨酰转移RNA结合活性
J Mol Biol. 1971 Sep 14;60(2):347-64. doi: 10.1016/0022-2836(71)90299-3.