Dubreuil P, Morisset J
Pancreas. 1986;1(1):13-9. doi: 10.1097/00006676-198601000-00004.
The current study examines the effects of somatocrinin (GRF) and somatostatin antiserum (ASS) alone and in combination on pancreatic growth. Twenty-four-day-old rats were injected daily s.c. at 10:00 and 16:30 h for 14 days with either saline or GRF (4 and 20 micrograms X kg-1). ASS was given i.p. every other day. Pancreatic weights and DNA, RNA, protein, amylase, and chymotrypsin total contents and concentrations were evaluated at the end of treatment. GRF alone was associated with significant decreases in pancreatic weight and contents of protein, amylase, and chymotrypsin but with significant increases in total DNA content indicating pancreatic atrophy and hyperplasia. ASS alone has a slight effect on DNA content but potentiates GRF given at the dose of 4 micrograms X kg-1. Even though ASS reduced protein and enzyme contents, it prevented the large decreases observed with increasing doses of GRF. These data present for the first time effects of GRF and ASS on pancreatic growth and describe a strong interaction between the two products. It is yet too early to determine how these two substances work on the pancreatic acinar cells, but hypotheses on their possible mode of action are proposed.
本研究探讨了生长激素释放因子（GRF）和生长抑素抗血清（ASS）单独及联合应用对胰腺生长的影响。将24日龄大鼠于每天10:00和16:30皮下注射，连续14天，注射生理盐水或GRF（4和20微克/千克）。ASS每隔一天腹腔注射。在治疗结束时评估胰腺重量以及DNA、RNA、蛋白质、淀粉酶和胰凝乳蛋白酶的总含量及浓度。单独使用GRF会导致胰腺重量以及蛋白质、淀粉酶和胰凝乳蛋白酶含量显著降低，但总DNA含量显著增加，表明胰腺萎缩和增生。单独使用ASS对DNA含量有轻微影响，但能增强4微克/千克剂量的GRF的作用。尽管ASS降低了蛋白质和酶的含量，但它阻止了随着GRF剂量增加而出现的大幅下降。这些数据首次呈现了GRF和ASS对胰腺生长的影响，并描述了这两种物质之间的强烈相互作用。确定这两种物质如何作用于胰腺腺泡细胞还为时过早，但提出了关于它们可能作用方式的假说。
