Satake K, Mukai R, Kato Y, Umeyama K
Pancreas. 1986;1(3):246-53. doi: 10.1097/00006676-198605000-00008.
The effects of cerulein on normal pancreas and on N-nitrobis (2-hydroxypropyl) amine (BHP)-induced experimental pancreatic carcinoma in Syrian golden hamsters were studied. Twenty hamsters received a subcutaneous injection of cerulein (20 micrograms/kg). The results showed that when cerulein was injected subcutaneously for 10 days, pancreatic weight and amylase increased. DNA and the pancreatic weight/DNA ratio were also increased significantly in treated hamsters compared with controls (p less than 0.02 versus p less than 0.01). These results indicated that chronic cerulein injection had hypertrophic and hyperplastic effects. DNA synthesis, as measured by histoautoradiography of tritiated thymidine-labeled tissue, increased in pancreatic acinar cells (p less than 0.01) and increased slightly in islet cells and in ductal cells. Tritiated thymidine uptake in the pancreas of the treated group indicated a rather selective exocrine gland incorporation by acinar rather than ductal cells. Sixty hamsters received a subcutaneous injection of BHP (500 mg/kg) once a week, while 63 hamsters received BHP (500 mg/kg) plus cerulein (20 micrograms/kg). Twenty-seven hamsters received cerulein (20 micrograms/kg) alone. All animals were killed from 8 to 27 weeks later, and no cancer-bearing hamsters were observed during the eighth and ninth week following administration. From the 10th to 14th weeks after administration of BHP and cerulein, 87.9% (13 of 15) had tumors compared with 18.7% (3 of 16) after BHP alone (p less than 0.01). One of three and two of 13 tumors were adenoma.(ABSTRACT TRUNCATED AT 250 WORDS)
研究了蛙皮素对叙利亚金黄地鼠正常胰腺以及对N-亚硝基双（2-羟丙基）胺（BHP）诱导的实验性胰腺癌的影响。20只仓鼠接受皮下注射蛙皮素（20微克/千克）。结果显示，皮下注射蛙皮素10天时，胰腺重量和淀粉酶增加。与对照组相比，经处理的仓鼠的DNA以及胰腺重量/DNA比值也显著增加（分别为p<0.02和p<0.01）。这些结果表明，长期注射蛙皮素具有肥大和增生作用。通过氚标记胸腺嘧啶核苷标记组织的组织放射自显影法测量，胰腺腺泡细胞中的DNA合成增加（p<0.01），胰岛细胞和导管细胞中的DNA合成略有增加。处理组胰腺中氚标记胸腺嘧啶核苷的摄取表明，腺泡细胞而非导管细胞对其有相当选择性的外分泌腺掺入。60只仓鼠每周接受一次皮下注射BHP（500毫克/千克），而63只仓鼠接受BHP（500毫克/千克）加蛙皮素（20微克/千克）。27只仓鼠单独接受蛙皮素（20微克/千克）。所有动物在8至27周后处死，给药后第8周和第9周未观察到患癌仓鼠。在给予BHP和蛙皮素后的第10至14周，87.9%（15只中的13只）出现肿瘤，而单独给予BHP后为18.7%（16只中的3只）（p<0.01）。16只肿瘤中有3只中的1只以及13只肿瘤中有2只为腺瘤。（摘要截取自250字）
