Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Shanghai, China.
J Sep Sci. 2014 Mar;37(5):515-26. doi: 10.1002/jssc.201301036. Epub 2014 Jan 22.
HPLC with diode array detection and ESI-TOF-MS was used for the study of the constituents in Apocynum venetum L. extracts and the metabolites in rat urine after oral administration of A. venetum L. extracts. A formula database of the known constituents in A. venetum L. was established, and 21 constituents were rapidly identified by accurately matching their molecular masses with the formulae of the compounds in the database. Furthermore, 34 metabolites were detected and elucidated in the rat urine. The scientific and plausible biotransformation pathways of the flavonoid components in A. venetum L. were also proposed together with the presentation of clues for potential mechanisms of bioactivity. This specific and sensitive HPLC-ESI-TOF-MS method can be used to identify the chemical components in the extracts of A. venetum L. and their metabolites in rat urine. This method can also be used to reveal the possible metabolic mechanisms of action of the extract components in vivo.
采用高效液相色谱二极管阵列检测-电喷雾飞行时间质谱联用技术研究了罗布麻提取物的化学成分以及灌胃给予罗布麻提取物后大鼠尿液中的代谢产物。建立了罗布麻已知成分的配方数据库，通过准确匹配化合物数据库中化合物的分子量，快速鉴定出 21 种成分。此外，还检测并阐明了大鼠尿液中的 34 种代谢产物。还提出了罗布麻中类黄酮成分的科学合理的生物转化途径，并提出了潜在生物活性机制的线索。该方法具有特异性和灵敏度高的特点，可用于鉴定罗布麻提取物中的化学成分及其在大鼠尿液中的代谢产物。该方法还可用于揭示提取物成分在体内的可能代谢作用机制。
