Kull F C, Brent D A, Parikh I, Cuatrecasas P
Science. 1987 May 15;236(4803):843-5. doi: 10.1126/science.2437656.
Neoplasms produce substances that induce blood vessel formation (angiogenesis). Fractions from ethanol extracts of the Walker 256 carcinoma were isolated by silica column chromatography and C18 reversed-phase high-performance liquid chromatography. Two of the isolated fractions induced neovascularization when tested in the rabbit corneal micropocket assay. One of the fractions was identified as nicotinamide by desorption-electron impact mass spectrometry, nuclear magnetic resonance spectroscopy, and gas chromatography-mass spectrometry. The second active fraction contained nicotinamide as part of a more complex, as yet unidentified, molecular arrangement. Microgram quantities of commercial nicotinamide induced neovascularization in the corneal micropocket assay and in the chick chorioallantoic membrane assay.
肿瘤会产生诱导血管形成（血管生成）的物质。通过硅胶柱色谱法和C18反相高效液相色谱法对沃克256癌乙醇提取物的馏分进行分离。在兔角膜微袋试验中测试时，其中两个分离出的馏分诱导了新血管形成。通过解吸电子轰击质谱、核磁共振光谱和气相色谱-质谱法，其中一个馏分被鉴定为烟酰胺。第二个活性馏分含有烟酰胺，它是一种更复杂、尚未确定的分子排列的一部分。微克量的商业烟酰胺在角膜微袋试验和鸡胚绒毛尿囊膜试验中诱导了新血管形成。
