ISEV 立场文件：细胞外囊泡 RNA 分析和生物信息学。
ISEV position paper: extracellular vesicle RNA analysis and bioinformatics.
机构信息
Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Parkville, Victoria, Australia.
Department of Pathology, VU University Medical Centre, Cancer Center Amsterdam, Amsterdam, The Netherlands.
出版信息
J Extracell Vesicles. 2013 Dec 23;2. doi: 10.3402/jev.v2i0.22859. eCollection 2013.
Abstract
Extracellular vesicles (EVs) are the collective term for the various vesicles that are released by cells into the extracellular space. Such vesicles include exosomes and microvesicles, which vary by their size and/or protein and genetic cargo. With the discovery that EVs contain genetic material in the form of RNA (evRNA) has come the increased interest in these vesicles for their potential use as sources of disease biomarkers and potential therapeutic agents. Rapid developments in the availability of deep sequencing technologies have enabled the study of EV-related RNA in detail. In October 2012, the International Society for Extracellular Vesicles (ISEV) held a workshop on "evRNA analysis and bioinformatics." Here, we report the conclusions of one of the roundtable discussions where we discussed evRNA analysis technologies and provide some guidelines to researchers in the field to consider when performing such analysis.
摘要
细胞外囊泡（EVs）是指细胞释放到细胞外空间的各种囊泡的统称。这些囊泡包括外泌体和微泡，它们通过大小和/或蛋白质和遗传物质的负载而有所不同。随着 EV 中含有 RNA（evRNA）的发现，人们对这些囊泡的潜在用途作为疾病生物标志物和潜在治疗剂的来源产生了越来越大的兴趣。高通量测序技术的快速发展使得对 EV 相关 RNA 的研究得以详细进行。2012 年 10 月，国际细胞外囊泡学会（ISEV）举办了一次关于“evRNA 分析和生物信息学”的研讨会。在这里，我们报告了其中一个圆桌讨论的结论，该讨论讨论了 evRNA 分析技术，并为该领域的研究人员提供了一些在进行此类分析时需要考虑的指导方针。
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