Department of Animal Science, Facultad de Ciencias Veterinarias, Universidad de Concepción, Avenida Vicente Méndez 595, Chillán, Chile.
Department of Animal Science, Facultad de Ciencias Veterinarias, Universidad de Concepción, Avenida Vicente Méndez 595, Chillán, Chile.
Res Vet Sci. 2014 Feb;96(1):95-101. doi: 10.1016/j.rvsc.2013.12.005. Epub 2013 Dec 13.
Repair of injured soft and hard tissues in horses can benefit greatly from the use of regenerative therapies with mesenchymal stem cells (MSC). Vitamin-C and platelet-rich-plasma had been used for in vitro differentiation of MSC. This study was aimed to evaluate the effect of vitamin-C, platelet-rich-plasma and their combination on the in vitro differentiation of adipose horse MSC. We isolated MSC from horse fat and differentiated them in vitro into osteogenic and chondrogenic lineages, as demonstrated by specific staining and RT-qPCR of selected genes. Combining vitamin-C and plasma-rich-platelet positively affected the ability of MSC to differentiate in vitro into mesodermal lineages during 14 days of culture; this effect was not as marked when differentiation was attempted for 21 days. This provides valuable information on the effect of combined use of these molecules in regenerative therapies and their potential application along stem cells for lesions of musculoskeletal tissue in sport horses.
在马中，受伤的软、硬组织修复可以极大地受益于使用间充质干细胞（MSC）的再生疗法。维生素 C 和富含血小板的血浆已被用于 MSC 的体外分化。本研究旨在评估维生素 C、富含血小板的血浆及其组合对脂肪来源的马 MSC 体外分化的影响。我们从马脂肪中分离出 MSC，并通过特定染色和选定基因的 RT-qPCR 证明它们在体外分化为成骨和成软骨谱系。在 14 天的培养过程中，维生素 C 和富含血小板的血浆的组合可积极影响 MSC 体外向中胚层谱系分化的能力；当尝试分化 21 天时，这种作用并不明显。这为这些分子联合使用在再生疗法中的效果以及它们与干细胞一起应用于运动马的肌肉骨骼组织损伤提供了有价值的信息。
