无诱导条件下高效表达荧光蛋白。
Uninduced high-yield bacterial expression of fluorescent proteins.
机构信息
Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
Program in Molecular Biophysics, Johns Hopkins University, Baltimore, MD 21218, USA.
出版信息
Anal Biochem. 2014 Mar 15;449:155-7. doi: 10.1016/j.ab.2013.12.027. Epub 2013 Dec 28.
Abstract
Here we introduce a fast, cost-effective, and highly efficient method for production of soluble fluorescent proteins from bacteria. The method does not require optimization and does not use isopropyl β-d-1-thiogalactopyranoside (IPTG) induction. The method relies on uninduced expression in the BL21-Gold (DE3) strain of Escherichia coli and yields large amounts (up to 0.4 μmol) of fluorescent protein from a 250-ml culture. This method is much simpler than published methods and can be used to produce any fluorescent protein that is needed in biomedical research.
摘要
在这里，我们介绍了一种从细菌中快速、经济高效且高效地生产可溶性荧光蛋白的方法。该方法不需要优化，也不使用异丙基 β-D-1-硫代半乳糖吡喃糖苷（IPTG）诱导。该方法依赖于大肠杆菌 BL21-Gold（DE3）菌株的非诱导表达，可从 250 毫升培养物中产生多达 0.4 μmol 的荧光蛋白。该方法比已发表的方法简单得多，可用于生产生物医学研究中所需的任何荧光蛋白。
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