Porta C, Devergne J C, Cardin L, Briand J P, Van Regenmortel M H
Laboratoire d'Immunochimie, Institut de Biologie Moléculaire et Cellulaire, Strasbourg, France.
Arch Virol. 1989;104(3-4):271-85. doi: 10.1007/BF01315549.
Ten monoclonal antibodies (McAbs) against cucumber mosaic virus (CMV) have been raised from fusion experiments performed after immunizing mice with different CMV antigens. Their reactivities with members of the three CMV serotypes, CMV-DTL, CMV-ToRS, and CMV-Co were tested in a double antibody sandwich format of enzyme immunosorbent assay (ELISA). Several of the McAbs were specific for different members of the CMV-DTL and CMV-ToRS groups while two allowed the detection of CMV-Co. By using a mixture of two McAbs, any member of the three major CMV serotypes could be detected in infected plant sap. One of the antibodies made it possible to discriminate between subunits and whole virions of CMV-D when it was used in ELISA simultaneously as coating antibody and as biotin-conjugate. McAbs were shown to be useful for quantifying the amount of CMV present in plant sap.
通过用不同的黄瓜花叶病毒（CMV）抗原免疫小鼠后进行融合实验，已获得了十种抗黄瓜花叶病毒的单克隆抗体（McAbs）。采用酶联免疫吸附测定（ELISA）的双抗体夹心形式，测试了它们与三种CMV血清型CMV-DTL、CMV-ToRS和CMV-Co成员的反应性。其中几种单克隆抗体对CMV-DTL和CMV-ToRS组的不同成员具有特异性，而有两种可用于检测CMV-Co。通过使用两种单克隆抗体的混合物，可以在感染的植物汁液中检测到三种主要CMV血清型的任何成员。当其中一种抗体在ELISA中同时用作包被抗体和生物素偶联物时，能够区分CMV-D的亚基和完整病毒粒子。结果表明，单克隆抗体可用于定量植物汁液中CMV的含量。
