水解初榨椰子油在伤口愈合过程中对NIH 3T3细胞系的增殖及环氧化酶-2表达的促进活性。
The Activity of Hydrolyzed Virgin Coconut Oil to Increase Proliferation and Cyclooxygenase-2 Expression towards on NIH 3T3 Cell Line in Wound Healing Process.
作者信息
Silalahi Jansen, Yuandani Yuandani, Meliala Dian Ika Perbina Br, Margata Linda, Satria Denny
机构信息
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Jalan Tri Dharma No. 5, Kampus USU, Medan, 20155, Indonesia.
Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Jalan Tri Dharma No. 5, Kampus USU, Medan, 20155, Indonesia.
出版信息
Open Access Maced J Med Sci. 2019 Oct 14;7(19):3164-3168. doi: 10.3889/oamjms.2019.804. eCollection 2019 Oct 15.
AIM
This study aims to determine the effect of hydrolysed virgin coconut oil (HVCO) to increase cell proliferation, COX-2 expression of NIH 3T3.
METHODS
The sample used was Virgin Coconut Oil (VCO). VCO was partially hydrolysed using lipase from (active on sn-1,3 position) to produce hydrolysed VCO (HVCO) composed of free fatty acids, 2-monoglycerides. Then acid value was determined. The effect of HVCO on proliferation was evaluated using the MTT method. Wound healing assay was established by a cell migration method, and COX-2 expression was determined using RT-PCR.
RESULTS
Acid value is 135.89 ± 0.12 mg NaOH/g oil and free fatty acids (FFA) is 48.50 ± 0.06%. The effect of HVCO 62.5 µg/mL on cell proliferation after 24h, 48h, and 72h incubation found as viable cells are 109.24 ± 0.52%; 118.26 ± 0.91% and 106.59 ± 0.74%. Percent of wound closed after 24 h and 48 h incubation are 69.94 ± 0.54% and 100.00 ± 0.00%, and expression of COX-2 increased from 1 (control) to 1.83 (HVCO).
CONCLUSION
The results suggest that HVCO is effective to increase cells proliferation and hence wound healing process.
目的
本研究旨在确定水解初榨椰子油（HVCO）对NIH 3T3细胞增殖及COX - 2表达的影响。
方法
使用的样品为初榨椰子油（VCO）。利用（作用于sn - 1,3位的）脂肪酶对VCO进行部分水解，以产生由游离脂肪酸、2 - 单甘油酯组成的水解VCO（HVCO）。然后测定酸值。采用MTT法评估HVCO对增殖的影响。通过细胞迁移法建立伤口愈合试验，并使用RT - PCR测定COX - 2表达。
结果
酸值为135.89±0.12 mg NaOH/g油，游离脂肪酸（FFA）为48.50±0.06%。在孵育24小时、48小时和72小时后，发现62.5 µg/mL的HVCO对细胞增殖的影响表现为活细胞分别为109.24±0.52%；118.26±0.91%和106.59±0.74%。孵育24小时和48小时后伤口闭合的百分比分别为 69.94±0.54%和100.00±0.00%，COX - 2的表达从1（对照）增加到1.83（HVCO）。
结论
结果表明，HVCO可有效促进细胞增殖，从而促进伤口愈合过程。
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