Gutierrez-Gonzalvez M G, Armas-Portela R, Stockert J C
J Microsc. 1987 Mar;145(Pt 3):333-40.
After the application of a ruthenium red (RR) solution to smears of chicken and human blood for 15 min, thrombocyte and leucocyte nuclei showed a blue-grey colour, contrasting with the red-stained erythrocyte nuclei. Extracellular matrix in frozen sections of cartilage showed the blue-grey colour after 1 h of staining. After the application of RR for a prolonged time (24 h), goblet cell mucin, granules of salivary glands and starch granules in Epon-embedded tissues were coloured blue-grey, blue-green and brown-green respectively; although they appeared red after shorter staining times. Microspectrophotometric measurements of differentially stained structures, and correlation with the spectral behaviour of a related ruthenium compound (ruthenium violet), are presented. The formation in situ of this latter compound by interaction of RR with certain substrates and the capacity of RR to distinguish different cellular structures are discussed.
将钌红（RR）溶液涂抹在鸡和人血涂片上15分钟后，血小板和白细胞核呈蓝灰色，与染成红色的红细胞核形成对比。软骨冰冻切片中的细胞外基质染色1小时后呈蓝灰色。长时间（24小时）应用RR后，杯状细胞粘蛋白、唾液腺颗粒和环氧树脂包埋组织中的淀粉颗粒分别染成蓝灰色、蓝绿色和棕绿色；尽管在较短染色时间后它们呈红色。本文给出了对不同染色结构的显微分光光度测量结果，以及与相关钌化合物（钌紫）光谱行为的相关性。讨论了RR与某些底物相互作用原位形成后一种化合物的过程，以及RR区分不同细胞结构的能力。
