Bertolesi G E, de Cidre L L, Stockert J C
Departamento de Biología, Facultad de Ciencias Exactas y Naturales, y Area de Investigación, Instituto de Oncología A. H. Roffo, Universidad de Buenos Aires, Argentina.
Acta Histochem. 1995 Oct;97(4):401-8. doi: 10.1016/S0065-1281(11)80065-6.
In this work we describe the formation and microscopical application of a fluorescent derivative of Ruthenium Red (RR) obtained by heating the dye in the presence of 1,10-phenanthroline (OP). The RR-OP reaction product showed absorption maxima at 416 and 444 nm and intense fluorescence emission at 578 nm under 440 nm exciting light. Neither RR nor OP solutions alone were fluorescent when excited at 440 nm. Using fluorescence microscopy, chicken blood cell smears stained 5 min with the RR-OP derivative showed the chromatin of erythrocyte nuclei with a bright orange fluorescence under violet-blue (436 nm) exciting light.
在本研究中，我们描述了通过在1,10-菲咯啉（OP）存在下加热钌红（RR）获得的一种荧光衍生物的形成及其显微镜应用。RR-OP反应产物在416和444nm处有吸收最大值，在440nm激发光下于578nm处有强烈的荧光发射。单独的RR溶液和OP溶液在440nm激发时均无荧光。使用荧光显微镜，用RR-OP衍生物染色5分钟的鸡血细胞涂片在紫蓝色（436nm）激发光下显示红细胞核染色质发出明亮的橙色荧光。
