Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Biosens Bioelectron. 2014 May 15;55:209-15. doi: 10.1016/j.bios.2013.12.007. Epub 2013 Dec 10.
In this paper, we report an efficient and high-performance immunoassay platform by combining high-density vertical ZnO nanowire array with photostable quantum dot (QD) labeling. The ZnO nanowire array provides a large surface area for the immobilization of biomolecules, which makes it an efficient substrate for the immunoreaction of biomolecules. When a sandwich immunoassay with QD label was conducted on various substrates, the ZnO nanowire substrate showed stronger fluorescence signal than ZnO thin film and bare glass substrates by 3.8 and 8.5 times, respectively. We found that the fluorescence resonance energy transfer (FRET) from QD to ZnO nanowire could be suppressed by extending their distance with multilayer biotin-streptavidin complex. In addition, we demonstrated the QD-based immunoassay of carcinoembryonic antigen (CEA) on a ZnO nanowire substrate, showing an excellent immunoassay performance with a very low detection limit (0.001 ng/mL) and a large detection range up to 100 ng/mL.
本文报道了一种高效、高性能的免疫分析平台，该平台结合了高密度垂直 ZnO 纳米线阵列和光稳定量子点 (QD) 标记。ZnO 纳米线阵列为生物分子的固定提供了一个大的表面积，使其成为生物分子免疫反应的有效基底。当在各种基底上进行带有 QD 标记的三明治免疫分析时，ZnO 纳米线基底的荧光信号比 ZnO 薄膜和裸玻璃基底分别强 3.8 倍和 8.5 倍。我们发现，通过多层生物素-链霉亲和素复合物延长 QD 与 ZnO 纳米线的距离，可以抑制荧光共振能量转移 (FRET)。此外，我们在 ZnO 纳米线基底上展示了基于 QD 的癌胚抗原 (CEA) 的免疫分析，具有非常低的检测限 (0.001ng/mL) 和高达 100ng/mL 的大检测范围的优异免疫分析性能。
