Department of Biology, College of Natural Science, Jeju National University, Jeju, 690-756, Republic of Korea.
J Microbiol. 2013 Dec;51(6):872-6. doi: 10.1007/s12275-013-2500-7. Epub 2013 Dec 19.
A Gram-positive, endospore-forming, rod-shaped bacterium, designated strain N2-5(T), was isolated from swinery waste collected in Jeju, Republic of Korea. A phylogenetic analysis based on 16S rRNA gene sequences demonstrated that strain N2-5(T) formed a phyletic group within the phylum Firmicutes with less than 97.0% similarities to members of the genus Lysinibacillus, its nearest phylogenetic neighbors. The highest levels of sequence similarity to the isolate were observed against Lysinibacillus xylanilyticus XDB9(T) (96.8%), Lysinibacillus macroides LMG 18474(T) (95.6%), and Lysinibacillus parviboronicapiens BAM-582(T) (95.6%). The organism grew optimally at 30°C and pH 7 and in the presence of 1-3% (w/v) NaCl. Strain N2-5(T) was chemotaxonomically characterized by possessing menaquinone-7 (MK-7) as the major menaquinone, and iso-C15:0 (54.9%), iso-C17:1 ω10c (12.0%), and C16:1 ω7c alcohol (11.8%) as the predominant fatty acids. The genomic DNA G+C content of the novel strain was 43.3 mol% and the cell-wall peptidoglycan was type A4α. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. Based on its phenotypic properties and phylogenetic data, strain N2-5(T) (=DSM 28310(T) =KCTC13837(T)) represents a novel species in the genus Lysinibacillus, for which the name Lysinibacillus jejuensis sp. nov. is proposed.
一株革兰氏阳性、产芽孢的杆状细菌，被命名为 N2-5(T) 菌株，从韩国济州岛收集的养猪场废物中分离得到。基于 16S rRNA 基因序列的系统发育分析表明，N2-5(T) 菌株在厚壁菌门内形成一个分支，与赖氨酸芽孢杆菌属的成员相似度低于 97.0%，是其最接近的系统发育近邻。与该分离株的序列相似性最高的是木聚糖赖氨酸芽孢杆菌 XDB9(T)（96.8%）、巨大芽孢杆菌 LMG 18474(T)（95.6%）和小硼酸赖氨酸芽孢杆菌 BAM-582(T)（95.6%）。该菌最适生长温度为 30°C，pH 值为 7，在 1-3%（w/v）NaCl 存在下生长。N2-5(T) 菌株的化学分类特征为主要的类异戊二烯醌为menaquinone-7 (MK-7)，主要脂肪酸为 iso-C15:0 (54.9%)、iso-C17:1 ω10c (12.0%)和 C16:1 ω7c 醇 (11.8%)。新菌株的基因组 DNA G+C 含量为 43.3 mol%，细胞壁肽聚糖为 A4α 型。主要的极性脂为双磷脂酰甘油、磷脂酰甘油和磷脂酰乙醇胺。根据其表型特性和系统发育数据，N2-5(T) 菌株（=DSM 28310(T)=KCTC13837(T)）代表赖氨酸芽孢杆菌属中的一个新种，现提议将其命名为济州岛赖氨酸芽孢杆菌（Lysinibacillus jejuensis）sp. nov.。
