Laboratory of Microbiology (LM-UGent), Department of Biochemistry and Microbiology, Ghent University, K.L. Ledeganckstraat 35, 9000 Ghent, Belgium.
Laboratory of Biochemistry and Brewing, Faculty of Applied Engineering Sciences, University College Ghent, Valentin Vaerwyckweg 1, 9000 Ghent, Belgium.
Int J Syst Evol Microbiol. 2012 May;62(Pt 5):1121-1127. doi: 10.1099/ijs.0.027995-0. Epub 2011 Jul 1.
'Bacillus macroides' ATCC 12905(T) ( = DSM 54(T) = LMG 18474(T)), isolated in 1947 from cow dung, was not included in the Approved Lists of Bacterial Names and so it lost standing in bacteriological nomenclature. Reinvestigation of the strain, including DNA-DNA relatedness experiments, revealed that 'Bacillus macroides' is genomically distinct from its closest relatives Lysinibacillus xylanilyticus, Lysinibacillus boronitolerans and Lysinibacillus fusiformis (as determined by 16S rRNA gene sequence analysis, with pairwise similarity values of 99.2, 98.8 and 98.5 %, respectively, with the type strains of these species). Further analysis showed that 'Bacillus macroides' shares the A4α L-Lys-D-Asp peptidoglycan type with other members of the genus Lysinibacillus and can thus be attributed to this genus. These results, combined with additional phenotypic data, justify the description of strain LMG 18474(T) ( = DSM 54(T) = ATCC 12905(T)) as Lysinibacillus macroides sp. nov., nom. rev.
“巨大芽孢杆菌”ATCC 12905(T)（=DSM 54(T)=LMG 18474(T)），于 1947 年从牛粪中分离得到，未列入《批准的细菌名称清单》，因此在细菌命名学中失去了地位。对该菌株的重新研究，包括 DNA-DNA 相关性实验，表明“巨大芽孢杆菌”在基因组上与其最接近的亲缘种解木聚糖芽孢杆菌、硼耐受芽孢杆菌和梭形芽孢杆菌不同（通过 16S rRNA 基因序列分析确定，与这些种的模式株的相似性值分别为 99.2、98.8 和 98.5%）。进一步的分析表明，“巨大芽孢杆菌”与其他赖氨酸芽孢杆菌属成员共享 A4α L-Lys-D-Asp 肽聚糖类型，因此可以归属于该属。这些结果与其他表型数据结合，证明了 LMG 18474(T)（=DSM 54(T)=ATCC 12905(T)）菌株作为赖氨酸芽孢杆菌属新种的描述是合理的，即赖氨酸芽孢杆菌巨大亚种，名称的重新认定。
