Vogel D W, Hartmann R K, Struck J C, Ulbrich N, Erdmann V A
Nucleic Acids Res. 1987 Jun 11;15(11):4583-91. doi: 10.1093/nar/15.11.4583.
From the gram-negative eubacterium Pseudomonas aeruginosa we have isolated a stable 6S RNA, approximately 180 nucleotides in length. The RNA was partially sequenced and identified by comparison with the known Escherichia coli 6S RNA sequence. Southern hybridizations revealed a single copy gene coding for the 6S RNA. DNA from other prokaryotes, i.e. E. coli, Thermus thermophilus, Bacillus subtilis, Bacillus stearothermophilus and Halobacterium maris mortui, did not give detectable hybridization signals. The 6S RNA gene was cloned in E. coli and its complete primary structure was determined. Although the 6S RNA sequences from P. aeruginosa and E. coli share only a 60.4% homology, we are able to propose a common secondary structural model.
我们从革兰氏阴性真细菌铜绿假单胞菌中分离出一种稳定的6S RNA，长度约为180个核苷酸。通过与已知的大肠杆菌6S RNA序列进行比较，对该RNA进行了部分测序和鉴定。Southern杂交显示存在一个编码6S RNA的单拷贝基因。来自其他原核生物（即大肠杆菌、嗜热栖热菌、枯草芽孢杆菌、嗜热脂肪芽孢杆菌和死海嗜盐菌）的DNA未给出可检测到的杂交信号。6S RNA基因被克隆到大肠杆菌中，并确定了其完整的一级结构。尽管铜绿假单胞菌和大肠杆菌的6S RNA序列仅具有60.4%的同源性，但我们仍能够提出一个共同的二级结构模型。
