Burger P C, Chandler D B, Fryczkowski A W, Klintworth G K
Scanning Microsc. 1987 Mar;1(1):223-31.
In light of the complicated nature of the ocular vasculature, it has been difficult to define the normal ocular anatomy by reference to two-dimensional tissue sections. Since it provides three-dimensional replicas, scanning electron microscopy (SEM) of vascular corrosion casts has therefore been an invaluable addition to the study of ocular vasculature. This technique also often permits identification of a normal vessel's arterial, venous, or capillary nature by its surface features. In addition, this technique is finding increased use in defining anatomical features of human vascular disease and is especially well suited for the study of experimental neovascularization as it relates to the eye. This paper reviews the application of SEM of microscopic casts to the study of normal and diseased ocular vessels, as well as the contribution of this method to studies of experimental ocular neovascularization.
鉴于眼部血管系统的复杂性，通过二维组织切片来定义正常眼部解剖结构一直很困难。由于扫描电子显微镜（SEM）对血管铸型进行扫描可提供三维复制品，因此它对眼部血管系统的研究而言是一项非常宝贵的补充技术。该技术还常常能够通过血管的表面特征来识别正常血管的动脉、静脉或毛细血管属性。此外，这项技术在定义人类血管疾病的解剖特征方面的应用越来越广泛，尤其适用于研究与眼睛相关的实验性新生血管形成。本文综述了微观铸型的扫描电子显微镜技术在正常和患病眼部血管研究中的应用，以及该方法对实验性眼部新生血管形成研究的贡献。
