Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung College of Health Sciences, Kaohsiung, Taiwan.
Department of Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University College of Medicine, Taipei, Taiwan.
J Microbiol Immunol Infect. 2015 Aug;48(4):397-405. doi: 10.1016/j.jmii.2013.11.006. Epub 2013 Dec 31.
Staphylococcus lugdunensis is a member of coagulase-negative staphylococci, which has the potential to cause serious infections, such as endocarditis, bone and joint infections, and septicemia. Differences in phenotypic/genotypic characterization may be linked to different diseases.
Genotypes of 11 S. lugdunensis isolates from bacteremia were determined by pulsed field gel electrophoresis and accessory gene regulator (agr) typing. The SCCmec elements in two oxacillin-resistant isolates were sequenced. Phenotypes were tested by antimicrobial susceptibility testing, biofilm formation assessments, and virulence factor analysis (hemolytic and protease activities).
Among the 11 isolates, six pulsotypes were found, and seven isolates belonged to two major pulsotypes. Two agr types (agr-1sl or agr-2sl) were found. The 11 isolates were susceptible to most antimicrobial agents tested. The SCCmec elements in two oxacillin-resistant isolates belonged to the SCCmec type V, but with additional ccrAB2 genes. The agr-2sl isolates (n = 7) displayed higher hemolytic and protease activities than the agr-1sl isolates. All isolates contained the icaA gene but with variable biofilm activities. The results suggest that protein might play an important part in S. lugdunensis biofilms, possibly through an ica-independent pathway. Of the 11 patients with S. lugdunensis bacteremia, one patient had a community-onset infection, and others had a hospital-acquired infection, which were mostly central venous catheter-related infections.
The 11 S. lugdunensis bacteremia isolates displayed various genotypes and phenotypes. Two oxacillin-resistant isolates contained SCCmec type V and carried additional ccrAB2 genes. Correlation of genotypes and phenotypes with infections needs further studies.
凝固酶阴性葡萄球菌是凝固酶阴性葡萄球菌的一员，它有可能导致严重的感染，如心内膜炎、骨和关节感染和败血症。表型/基因型特征的差异可能与不同的疾病有关。
通过脉冲场凝胶电泳和辅助基因调节（agr）型对来自菌血症的 11 株 S. lugdunensis 分离株的基因型进行了测定。对两株耐苯唑西林分离株中的 SCCmec 元素进行了测序。通过药敏试验、生物膜形成评估和毒力因子分析（溶血和蛋白酶活性）来检测表型。
在 11 株分离株中，发现了 6 种脉冲型，7 株分离株属于两种主要的脉冲型。发现了两种 agr 型（agr-1sl 或 agr-2sl）。11 株分离株对大多数测试的抗菌药物均敏感。两株耐苯唑西林分离株中的 SCCmec 元素属于 SCCmec 型 V，但带有额外的 ccrAB2 基因。agr-2sl 分离株（n = 7）的溶血和蛋白酶活性高于 agr-1sl 分离株。所有分离株均含有 icaA 基因，但生物膜活性不同。结果表明，蛋白质可能在 S. lugdunensis 生物膜中发挥重要作用，可能通过非 ica 途径。在 11 例 S. lugdunensis 菌血症患者中，1 例为社区获得性感染，其余为医院获得性感染，多为中心静脉导管相关性感染。
11 株 S. lugdunensis 菌血症分离株显示出不同的基因型和表型。两株耐苯唑西林分离株含有 SCCmec 型 V，并携带额外的 ccrAB2 基因。基因型和表型与感染的相关性需要进一步研究。
